The degradation of 2-chlorophenol (2-CP), 2,4-dichlorophenol (2,4-DCP), 2,4,6-trichlorophenol (2,4,6-TCP) and pentachlorophenol (PCP) via biological, advanced oxidative process (AOP) and sequential biological-AOP was investigated in this work. The white-rot fungus Trametes pubescens was used for the biodegradation of chlorophenols, while in AOP TiO2/UV was used. In the biological degradation, the effect of glucose as a cofactor was also evaluated. The highest degradations were obtained when the reaction medium was supplemented with glucose, ranging from 94.6% to 37.8%, with degradation activity for 2-CP > 2,4-DCP > PCP > 2,4,6-TCP. During the AOP the removal initial rate increased in the following order 2-CP > 2,4,6-TCP > 2.4-DCP > PCP, and the obtained degradation range from 82.0% to 24.0%. When biological removal process, Supplemented with glucose, was followed for an AOP process, 100% degradation was obtained for all the chlorophenols tested. These results suggest that the white-rot fungi T pubescens could be used for the degradation of xenobiotic compounds, and its use with an advanced oxidative process, in a sequential mode, may be considered to obtain a complete removal of them. (c) 2010 Elsevier Ltd. All rights reserved.