The aim of the present study was to: (1) identify the nature of fouling for ED membranes (AMX and CMB, from Tokuyama Soda, Japan) used for the isolation and concentration of total NH(3)-N from swine manure, (2) determine the effect of fouling on membrane integrity, (3) establish the relation between fouling type and manure composition, and (4) estimate the efficiency of a two-step cleaning procedure to restore membranes properties. After processing 10 batches of swine manure (or 240 L/m(2)), the average current density as well as the membranes electrical conductivity and ion-exchange capacity decreased. The decline in process performance was associated with membrane fouling, since a significant deposit, possibly calcium carbonate and silica colloidal particles, was observed on the fouled AMX membranes. The electrical conductivity and ion-exchange capacity of the CMB membrane was completely restored by a two-step cleaning procedure using 0.5% NaOH and 1% HCI. However, for the electrical conductivity of the AMX membranes it was only partially recovered. The on-line cleaning procedure efficiency was assessed by measuring the stack average current density and the decrease of manure conductivity during I h tests. Values for the cleaned membranes were, respectively, 95% and 91% the ones measured with the new membranes, and were significantly higher than for the fouled membranes. Crown Copyright (c) 2008 Published by Elsevier Ltd. All rights reserved.