In this study, various Artificial Neural Networks (ANNs) were developed to estimate the production yield of greenhouse basil in Iran. For this purpose, the data collected by random method from 26 greenhouses in the region during four periods of plant cultivation in 2009-2010. The total input energy and energy ratio for basil production were 14,308,998 MJ ha(-1) and 0.02, respectively. The developed ANN was a multilayer perceptron (MLP) with seven neurons in the input layer, one, two and three hidden layer(s) of various numbers of neurons and one neuron (basil yield) in the output layer. The input energies were human labor, diesel fuel, chemical fertilizers, farm yard manure, chemicals, electricity and transportation. Results showed, the ANN model having 7-20-20-1 topology can predict the yield value with higher accuracy. So, this two hidden layer topology was selected as the best model for estimating basil production of regional greenhouses with similar conditions. For the optimal model, the values of the models outputs correlated well with actual outputs, with coefficient of determination (R-2) of 0.976. For this configuration, RMSE and MAE values were 0.046 and 0.035, respectively. Sensitivity analysis revealed that chemical fertilizers are the most significant parameter in the basil production. (C) 2011 Elsevier Ltd. All rights reserved.