An integrated detection circuitry based on a lock-in amplifier was designed for contactless conductivity determination of heavy metals. Combined with a simple-structure electrophoresis microchip, the detection system is successfully utilized for the separation and determination of various heavy metals. The influences of the running buffer and detection conditions on the response of the detector have been investigated. Six millimole 2-morpholinoethanesulfonic acid + histidine were selected as buffer for its stable baseline and high sensitivity. The best signals were recorded with a frequency of 38 kHz and 20 Vpp. The results showed that Mn2+, Cd2+, Co2+, and Cu2+ can be successfully separated and detected within 100 s by our system. The detection limits for five heavy metals (Mn2+, Pb2+, Cd2+, Co2+, and Cu2+) were determined to range from about 0.7 to 5.4 mu M. This microchip system performs a crucial step toward the realization of a simple, inexpensive, and portable analytical device for metal analysis.