Due to recent reports on toxicity of hindered-amine light stabilizers (HALS) in polymer materials, demand for the regulation of the dosage of HALS is increasing. Therefore development of a quantitation method for low concentration of HALS is required either for toxicity study of HALS in water or saliva extracts from polymeric materials or for accurate determination of reduced amounts of HALS in polymeric materials. Here we present a novel quantitation method for Tinuvin 292, a worldwide used HALS, in acrylic resins using high-performance liquid chromatography-electrospray ionization-ion trap mass spectrometry at a broad concentration ranging from 10 ppb to 50 ppm. Collision-induced dissociation was performed using helium gas, and the multiple reaction monitoring parameters were optimized for detection of parent/daughter ions of Tinuvin 292. Sample preparation employed a very simple extraction of Tinuvin 292 from acrylic resins with considerable recovery. The method was successfully applied to quantitate Tinuvin 292 in various acrylic polymers.