Shrink-Film Configurable Multiscale Wrinkles for Functional Alignment of Human Embryonic Stem Cells and their Cardiac Derivatives

Chen A; Lieu DK; Freschauf L; Lew V; Sharma H; Wang JX; Nguyen D; Karakikes I; Hajjar RJ; Gopinathan A; Botvinick E; Fowlkes CC; Li RA; Khine M

Advanced Materials, Vol.23, No.48, 5785-5785, 2011

DOI10.1002/adma.201103463 Export Citation

Shrink-Film Configurable Multiscale Wrinkles for Functional Alignment of Human Embryonic Stem Cells and their Cardiac Derivatives

Chen A, Lieu DK, Freschauf L, Lew V, Sharma H, Wang JX, Nguyen D, Karakikes I, Hajjar RJ, Gopinathan A, Botvinick E, Fowlkes CC, Li RA, Khine M

A biomimetic substrate for cell-culture is fabricated by plasma treatment of a prestressed thermoplastic shrink film to create tunable multiscaled alignment "wrinkles". Using this substrate, the functional alignment of human embryonic stem cell derived cardiomyocytes is demonstrated.

Keywords:biomimetics;human embryonic stem cell derived cardiomyocytes;contact guidance;action potential propagation