Three novel conjugated polymers based on 2,2'-biimidazole have been successfully designed and synthesized through the Suzuki coupling reactions, and their fluorescence sensing ability to metal ions and anions was investigated. The emission of the two polymers with hydrophilic side chains can be efficiently quenched by Cu2+ through a photoinduced electron transfer process. Moreover, the polymer Cu2+ complexes exhibit excellent "turn on" sensing properties for detection of pyrophosphate (PP anion. These complex sensors possess high selectivity avoiding the interference from other anions, very fast response (less than 3 min) to PPi, and the detection limit of about 0.17 ppm. In addition, the linear detection range of PPi can be tuned conveniently by changing the amount of Cu2+ ions. Thus, the conjugated polymers can be used as a novel fluorescent sensing platform, and this work provides a new strategy for the development of PPi sensors.