Three-pole electrospinning devices integrated with a blade-cage collector were developed to fabricate well-aligned nano-fiberous membranes. The proposed three-pole configuration with a channel electrode can be a powerful tool in aligning nano-fibers with regular diameter because the generated electric field can be accurately controlled without severe fluctuation in comparison with other methods. The three-pole electrospinning method is also valid for industrial mass production and accurate diameter control of the aligned nano-fibers.