There has been enormous interest in the last decade in development methods for the inorganic synthesis of metallic nanopartides of desired sizes and shapes because of their unique properties and extensive applications in catalysis, electronics, plasmonics, and sensing. Here we report on an environmentally friendly, one-pot synthesis of metallic nanoparticles, which avoids the use of organic solvents and requires mild experimental conditions. The developed method uses liposomes as nanoreactors, where the liposomes were prepared by encapsulating chloroauric acid and exploited the use of glycerol, incorporated within the lipid bilayer as well as in its hydrophilic core, as a reducing agent for the controlled preparation of highly homogeneous populations of gold nanopartides. The effects of temperature, the presence of a capping agent, and the concentration of glycerol on the size and homogeneity of the nanoparticles formed were investigated and compared with solution-based glycerol-mediated nanoparticle synthesis. Well-distributed gold nanoparticle populations in the range of 2-8 nm were prepared in the designed liposomal nanoreactor with a dear dependence of the size on the concentration of glycerol, the temperature, and the presence of a capping agent whereas large, heterogeneous populations of nanopartides with amorphous shapes were obtained in the absence of liposomes. The particle morphology and sizes were analyzed using transmission electron microscopy imaging, and the liposome size was measured using photon correlation spectroscopy.