Molecular dynamics simulations were performed for electro-osmotic flow (EOF) confined in a polyelectrolyte-grafted nanochannel under variable grafting density and normal electric field. With decreasing the value of the normal electric field, the brush undergoes a collapse transition, and the ion distribution is changed significantly. The brush thickness increases on increasing the grafting density at positive and weak negative electric fields, whereas a reduced brush thickness is observed at strong negative electric field. Our results further reveal that the flow velocity is not only dependent on conformational transition of the brush but also related to the cation and anion distributions. At low grafting density, the EOF is almost completely quenched at high electric field strength due to strong surface friction between ions and walls. For the case of very dense grafting, the flow velocity is influenced weakly within the brush when varying the grafting density. Additionally, a bidirectional flow occurs at an intermediate electric field. The investigation on fluid flux indicates that the fluid flux is insensitive to the grafting density, when the normal electric field is removed. For nonzero normal electric fields, a significant change in the fluid flux is observed at low grafting densities. (C) 2012 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys, 2012