In this paper it is shown that, for multi-input single-output non-affine non-linear systems, when a state feedback control stabilizes an equilibrium point of a plant with a certain bounded region of attraction, it is also stabilized by an output feedback controller with arbitrarily small loss of the region. Moreover, the proposed output feedback controller has the dynamic order n which is the same as the order of the plant. From any given state feedback, an explicit form of the overall controller is provided. A sufficient condition presented for the result is shown to be necessary and sufficient for regional uniform observability when the system is input affine. Thus, the result can be regarded as a regional separation principle for affine non-linear systems.