화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.36, No.4, 420-426, July, 2012
Export Citation
Quercetin과 Rutin을 함유하는 PCL-b-PEG 고분자 미셀의 특성 및 피부 흡수에 관한 In vitro 연구
Physical Characteristic and In vitro Transdermal Delivery of PCL-b-PEG Micelles Containing Quercetin and Rutin
임규남, 김선영, 김민지, 박수남†
  • 서울과학기술대학교 정밀화학과, 화장품종합기술연구소
Gyu Nam Lim, Sun Young Kim, Min Ji Kim, and Soo Nam Park†
  • Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology, 232 Gongreung-ro, Nowon-gu, Seoul 139-743, Korea
E-mail:
초록
본 연구에서는 항산화 물질로 잘 알려진 quercetin과 그 배당체인 rutin을 함유하는 poly(ε-caprolactone)-bpoly(ethylene glycol) 미셀을 제조하여, 활성물질(quercetin, rutin)의 in vitro 피부 흡수 증진에 관한 연구를 수행하였다. 입자크기는 PCL-b-PEG 고분자의 농도가 증가함에 따라 미셀의 초기 입자 크기가 증가하는 경향을 보였다. 고분자 미셀의 표면 전위(Zeta potential)는 비교적 일정함을 확인하였다. 제조한 고분자 미셀의 피부 흡수력을 평가하기 위하여, 용액 상태의 활성물질을 미셀의 대조군으로 하여 Franz cell을 이용한 투과실험을 진행한 결과 용액 상태보다 미셀에서 더 높게 나타났음을 확인하였다. 또한 화장품 소재로서의 안전성 평가를 위한 인체 피부 일차자극 실험(patch test) 결과 어떠한 피부 자극도 관찰되지 않았다.
In this study, we prepared polymer micelles containing quercetin and rutin, known as antioxidants, using poly(ε-caprolactone)-b-poly(ethylene glycol), and evaluated in vitro skin permeation of the active materials. Quercetin and rutin loaded micelles were characterized by DSC (differential scanning calorimetry), HPLC (high performance liquid chromatography) and DLS (dynamic light scattering) measurements. The particle size of the polymer micelles increased in a concentration dependent manner (0.5~2.0% PCL-b-PEG). The Zeta potential of quercetin and rutin loaded micelles remained constant. To evaluate the skin penetration of PCL-b-PEG micelles, Franz diffusion cell experiment was performed. The aqueous solutions of quercetin and rutin were used as the control groups. Quercetin and rutin loaded PCL-b-PEG micelles showed more efficient skin permeation than the control groups. Safety assessment (patch test) of quercetin and rutin loaded PCL-b-PEG micelles on skin was performed to test application possibility of the polymer micelles to cosmetics. Any adverse symptoms were not observed.
Keywords:PCL-b-PEG;micelle;drug delivery system;quercetin;rutin.
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