Digestive enzymes characterization of krill (Euphausia superba) residues deoiled by supercritical carbon dioxide and organic solvents

Abdelkader Ali-Nehari; Seon-Bong Kim; Yang-Bong Lee; Byung-Soo Chun

Journal of Industrial and Engineering Chemistry, Vol.18, No.4, 1314-1319, July, 2012

DOI10.1016/j.jiec.2012.01.026 Export Citation

Digestive enzymes characterization of krill (Euphausia superba) residues deoiled by supercritical carbon dioxide and organic solvents

Abdelkader Ali-Nehari, Seon-Bong Kim¹, Yang-Bong Lee¹, and Byung-Soo Chun^{1, †}

International Program of Fisheries Sciences, Faculty of Fisheries Sciences, Pukyong National University, 45 Yongso-ro, Nam-Gu, Busan 608-737, Republic of Korea
¹Department of Food Science and Technology, Faculty of Fisheries Sciences, Pukyong National University, 45 Yongso-ro, Nam-Gu, Busan 608-737, Republic of Korea

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In this study, Enzyme activities of krill were characterized before and after lipid extraction by supercritical carbon dioxide (SC-CO2) and organic solvent, n-hexane and acetone. Krill SC-CO2 extraction was performed under the conditions of temperature range from 35 to 45 ℃ and pressure, 150-250 bar for 2.5 h with a constant flow rate of 22 g/min. Extraction yields of lipids increased with pressure and temperature. The digestive enzyme activities of protease, lipase and amylase of SC-CO2 treated krill residues were slightly decreased comparing to organic solvent, n-hexane and acetone treated residues. In SC-CO2 treated samples, all of the digestive enzymes showed slightly higher temperature stability. In the other hand the crude extracts of SC-CO2 and n-hexane treated krill samples showed almost same optimum pH and pH stability for each of the digestive enzymes. It was also found in SDS-PAGE that there are no significant differences in protein patterns of the crude extracts of untreated, SC-CO2, n-hexane and acetone treated krill indicating no denaturation of proteins.

Keywords:Krill;Digestives enzymes;Supercritical carbon dioxide extraction;n-Hexane;Acetone

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[1] Fereidoon S, Janak K, Trends Food Sci. Technol., 12, 435 (2001)

[2] Wasserman BP, Food Technol., 44(4), 118 (1990)

[3] Haard NF, Food Technol., 53(7), 64 (1998)

[4] De-Vecchi SD, Coppes Z, J. Food Biochem., 20, 193 (1996)

[5] Anwar A, Saleemuddin M, Bioresour. Technol., 6, 175 (1998)

[6] Vulfson EN, Industrial Application of Lipases, Cambridge University Press, Great Britain, 271 (1994)

[7] Oudjeriouat N, Moreau Y, Santimone M, Svensson B, Marchis-Mouren G, Desseaux V, Eur. J. Biochem., 270, 3871 (2003)

[8] Tou JC, Jaczynski J, Chen YC, Nutr. Rev., 65(2), 63 (2007)

[9] Bligh EG, Dyer WJ, Can. J. Biochem. Physiol., 37, 911 (1959)

[10] Pariser ER, Wallerstei MBn, Corkery CJ, Brown NL, Fish Protein Concentrate:Panacea for World Malnutrition, MIT Press, Cambridge, MA (1978)

[11] Sahena F, Zaidul ISM, Jinap S, Karim AA, Abbas KA, Norulaini NAN, Omar AKM, J. Food Eng., 95, 240 (2009)

[12] Yamaguchi K, Murakami M, Nakano H, Konosu, S, Kokura, H. Yamamoto H, Kosaka M, Hata K, J. Agric. Food Chem., 34, 904 (1986)

[13] Oda K, Murao S, Agric. Biol. Chem., 38, 2435 (1974)

[14] Vorderwulbcke T, Kieslich K, Erdmann H, Enzyme Microb. Technol., 14, 631 (1992)

[15] Hatzinikolaou DG, Kourentzi E, Stamatis H, Christakopoulos P, Kolisis FN, Kekos D, Macris BJ, J. Biosci. Bioeng., 88(1), 53 (1999)

[16] Miller GL, Anal. Chem., 31, 426 (1959)

[17] Laemmli UK, Nature., 227, 680 (1970)

[18] Morita A, Kajimoto O, J. Phys. Chem., 94, 6420 (1990)

[19] Bulgarevich DS, Sako T, Sugeta T, Otake K, Takebayashi Y, Kamizawa C, Horikawa Y, Kato M, Ind. Eng. Chem. Res., 41(9), 2074 (2002)

[20] Wimmer Z, Zarevucka M, Int. J. Mol. Sci., 11(1), 233 (2010)

[21] Giessauf A, Magor W, Steinberger DJ, Marr R, Enzyme Microb. Technol., 24(8-9), 577 (1999)

[22] Kamat SV, Beckman EJ, Russel AJ, Crit. Rev. Biotechnol., 15, 41 (1995)

[23] Habulin M, Knez Z, J. Chem. Technol. Biotechnol., 76(12), 1260 (2001)

[24] Eshel A, Lindner P, Smirnoff P, Newton S, Harpaz S, Comp. Biochem. Physiol., 627(A), 106 (1993)

[25] Natalia Y, Hashim R, Ali A, Chong A, Aquaculture., 233, 305 (2004)

[26] Gjellesvik DR, Lombardo D, Walther BT, Biochim. Biophys. Acta., 1124, 123 (1992)

[27] Munilla-Mordn R, Saborido-Rey F, Comp. Biochem. Physiol., 827(B), 113 (1996)

[28] Prasertsan P, Jitbunjerdkul S, Trairatananukoon T, Augur T (Eds.), New Horizons in Biotechnology, IRD editions, Kluwer Academic Publisher, India (2001)

[29] Abe R, Chib AY, Nakajima T, AAPG Bull., (11S), 197, 57 (2002)

[30] Stahl E, Quirin KW, Blagrove RJ, J. Agric. Food Chem., 32, 938 (1984)