With seven monomers (four polyethylene glycol (PEG) and three amine monomers) chosen as "winners" from a previous high throughput study, we have refined and improved their grafting, filtration and anti-fouling characteristics. To do this, we varied the composition of a series of alcohol/water mixtures that was characterized by their solubility parameters (delta(T)). for monomer solvents during grafting. A novel high throughput (HT) approach allowed us to select conditions with high statistical confidence for photo-graft polymerization (PGP) (i.e., best solvent and monomer) that produced the highest degree of grafting, intermediate permeation flux and sieving values, and the lowest fouling index values. These super protein-resistant graft modified synthetic membrane surfaces are synthesized from polyethylene glycol (PEG-300) in water (delta(T) = 47.8 MPa(1/2)) and N-isopropylacrylamide (NIPA) grafted in ethanol/water (50 wt%/50 wt%) mixture (delta(T) =35.9 MPa(1/2)), respectively. They exhibited very low protein fouling (R=0.31 +/- 0.06 and 0.28 +/- 0.16), very good relative flux values (or normalized resistances, R(w.mod)/R(initial) = 1.06 +/- 0.08 and 1.89 +/- 0.31) and increased bovine serum albumin (BSA) sieving, respectively. Besides identifying a variety of grafting conditions for PEG and amine monomers that allow one to select filtration performance of protein feeds among various competing trade-offs (maximize flux, retention and antifouling), we also demonstrate how HT-PGP is used to optimize two particular variables, solvent type and concentration, with previously identified monomer candidates. (C) 2010 Published by Elsevier B.V.