The anti-biofouling properties, le the resistance to protein adsorption and the inhibition of bacterial adhesion (biofilm formation) are of vital importance to separation membranes Surface hydrophilization is thought to be the most common approach to improve these performances for hydrophobic polymer membranes, such as microporous polypropylene membrane (MPPM) In this work. MPPM was highly hydrophilized by the UV-induced grafting of polysulfobetaine methacrylate (polySBMA), an outstanding representative of zwitterionic anti-/non-fouling polymers A sequential grafting strategy was improved by combining the conventional benzophenone entrapment and covalent immobilization methods FT-IR/ATR, XPS and FESEM were used to characterize the detailed surface structures of MPPM The hydrophilization effect was evaluated by water contact angle (WCA) and pure water flux measurements Besides, both protein filtration and initial adhesion of bacteria were performed to investigate the anti-biofouling properties of the modified MPPM The surface hydrophobicity of the bacteria was also measured and correlated with the adhesion results It is found that the Improved strategy allows the grafting to take place on the external surface and the grafting density to be well controlled by modulating the SBMA concentration The surface hydrophilicity of MPPM is significantly enhanced, characterized by a decrease of WCA from 145 to similar to 15 degrees and an increase of flux by 4 times These hydrophilized MPPM has strong resistance to protein fouling, having a flux recovery ratio higher than 95% under optimized conditions The initial adhesion of bacteria on the MPPM can be completely suppressed when the grafting density reaches 560 mu g/cm(2), regardless of the bacterial surface hydrophobicity (C) 2010 Elsevier B V All rights reserved.