This paper presents the permeation properties of monodispersed PMMA and silica particles with an average diameter of 150 and 200 nm, respectively, through MF membranes in dead-end mode under various conditions. In this study, it has been elucidated that the zeta potential of particles is one of the most important factors in determinating the permeation properties of PMMA and silica particles through MF membranes; larger repulsive force between the membrane and particles or between the particles themselves prevent particles from adhering onto the membrane surface, resulting in excellent permeation being maintained in the case of the silica particles. Furthermore, by conducting permeation tests systematically under various experimental conditions, it has also been shown that it is important for maintaining good permeation of particles to set conditions in which the number of particles which access each pore per certain time is sufficiently small, since particles cannot permeate through the membrane once the membrane is fouled by rejected particles.