Enzymatic assay method for measuring mizoribine levels in serum

Hiramitsu T; Ota H; Watarai Y; Achiha M; Fukami H; Sakasegawa S; Hino E; Yamaguchi T; Ueda S; Kagimoto Y; Tamura T; Uchida K

Journal of Bioscience and Bioengineering, Vol.112, No.2, 205-207, 2011

DOI10.1016/j.jbiosc.2011.03.017 Export Citation

Enzymatic assay method for measuring mizoribine levels in serum

Hiramitsu T, Ota H, Watarai Y, Achiha M, Fukami H, Sakasegawa S, Hino E, Yamaguchi T, Ueda S, Kagimoto Y, Tamura T, Uchida K

A sensitive and specific method for assaying serum mizoribine levels that can be applied to general automatic clinical analyzers was developed. Regression analysis of the enzymatic assay (y) vs. the HPLC method (x) produced the following relation: y = 0.964x + 0.090 (n = 262, Sy, x = 6.37 ng/mL). (C) 2011, The Society for Biotechnology, Japan. All rights reserved.

Keywords:Mizoribine;Drug monitoring;Inosine 5 '-monophosphate dehydrogenase;Nucleoside kinase;mizoribine 5 '-monophosphate