Development of a recombinant epsilon-poly-L-lysine synthetase expression system to perform mutational analysis

Yamanaka K; Kito N; Kita A; Imokawa Y; Maruyama C; Utagawa T; Hamano Y

Journal of Bioscience and Bioengineering, Vol.111, No.6, 646-649, 2011

DOI10.1016/j.jbiosc.2011.01.020 Export Citation

Development of a recombinant epsilon-poly-L-lysine synthetase expression system to perform mutational analysis

Yamanaka K, Kito N, Kita A, Imokawa Y, Maruyama C, Utagawa T, Hamano Y

epsilon-Poly-L-lysine (epsilon-PL) synthetase (Pls), which is a membrane protein with adenylation and thiolation domains characteristic of the nonribosomal peptide synthetases, catalyzes polymerization of L-lysine molecules (25-mer to 35-mer). Here, we report on the development of a recombinant Pls expression system that allowed us to perform a site-directed mutational analysis. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.

Keywords:epsilon-Poly-L-lysine;Biopolymer;Non-ribosomal peptide synthetase;Membrane protein;Streptomyces albulus