A sensitive hydroxylamine sensor was developed based on electrodeposition of Pt nanoclusters on choline film modified glassy carbon electrode (nano-Pt/Ch/GCE). The properties of the composites were characterized by field emission scanning electron microscope, X-ray photoelectron spectroscopy, powder X-ray diffraction, and electrochemical investigations. The designed nano-Pt/Ch/GCE showed a high sensing performance for hydroxylamine in a wide concentration ranges of 5.0 x 10(-7)-1.1 x 10(-3) M and 1.1 x 10(-3)-19 x 10(-3) M. The detection limit was 0.07 mu M (s/n = 3). The proposed electrode presented excellent operational and storage stability for the determination of hydroxylamine. Moreover, the sensor showed good sensitivity, selectivity, and reproducibility properties. All the results indicated the designed sensor had a good potential application in the determination of hydroxylamine.