One-pot conversion of levan prepared from Serratia levanicum NN to difructose anhydride IV by Arthrobacter nicotinovorans levan fructotransferase

Kikuchi H; Sakurai H; Nagura T; Aritsuka T; Tomita F; Yokota A

Journal of Bioscience and Bioengineering, Vol.109, No.3, 240-243, 2010

DOI10.1016/j.jbiosc.2009.09.041 Export Citation

One-pot conversion of levan prepared from Serratia levanicum NN to difructose anhydride IV by Arthrobacter nicotinovorans levan fructotransferase

Kikuchi H, Sakurai H, Nagura T, Aritsuka T, Tomita F, Yokota A

The newly established difructose anhydride IV (DFA IV) production system is comprised of the effective production of levan from sucrose by Serratia levanicum NN, the conversion of the levan into DFA IV by levan fructotransferase from Arthrobacter nicotinovorans GS-9, which is highly expressed in an Escherichia coli transformant, and a practical purification step. The chemical properties of DFA IV were also investigated. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.

Keywords:beta-D-fructofuranose-beta-D-fructofuranose-2,6 ':6,2 '-dianhydride DFA IV;Levan;Sucrose;Levan fructotransferase;Arthrobacter nicotinovorans;Serratia levanicum