Characterization of Aspergillus oryzae glycoside hydrolase family 43 beta-xylosidase expressed in Escherichia coli

Suzuki S; Fukuoka M; Ookuchi H; Sano M; Ozeki K; Nagayoshi E; Takii Y; Matsushita M; Tada S; Kusumoto KI; Kashiwagi Y

Journal of Bioscience and Bioengineering, Vol.109, No.2, 115-117, 2010

DOI10.1016/j.jbiosc.2009.07.018 Export Citation

Characterization of Aspergillus oryzae glycoside hydrolase family 43 beta-xylosidase expressed in Escherichia coli

Suzuki S, Fukuoka M, Ookuchi H, Sano M, Ozeki K, Nagayoshi E, Takii Y, Matsushita M, Tada S, Kusumoto KI, Kashiwagi Y

This is the first report of glycoside hydrolase family 43 beta-xylosidase from Aspergillus oryzae. To characterize this enzyme, the recombinant enzyme was expressed in Escherichia coli. Unlike known beta-xylosidases from fungal origins, the enzyme did not show substrate ambiguity and was stable at alkaline pH. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.

Keywords:Aspergillus oryzae;beta-Xylosidase;Glycoside hydrolase family 43;Recombinant enzyme;Wheat bran