Journal of Bioscience and Bioengineering, Vol.109, No.1, 73-74, 2010
A mass separation of chondrocytes from cartilage tissue utilizing an automatic crushing device
Porcine articular cartilage tissue was crushed using Multi-Beads-Shocker (TM) and digested with trypsin and collagenase type II. Chondrocyte cell yield was maximized by automatic crushing for 10 s, which was 4 times that by manual dicing with a surgical blade and might be useful for the automation of the cell processing. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.