Inorganic Chemistry, Vol.38, No.26, 5999-6002, 1999
Solid-phase synthesis and photophysical properties of DNA labeled at the nucleobase with Ru(bpy)(2)(4-m-4 '-pa-bpy)(2+)
A facile procedure for incorporating a Ru(diimine)(3)(2+) complex at the nucleobase in an oligonucleotide is reported that combines the advantages of Pd(0) cross-coupling and solid-phase DNA chemistries. These ruthenium-modified oligonucleotides form stable duplexes, and the favorable photophysical properties associated with the Ru(diimine)(3)(2+) complex are retained after site-specific covalent attachment.
Keywords:SEQUENCE-SPECIFIC CLEAVAGE;RESONANCE ENERGY-TRANSFER;ELECTRON-TRANSFER;NUCLEIC-ACID;OLIGONUCLEOTIDES;COMPLEXES;CHEMISTRY;RNA;INHIBITORS;TEXAPHYRIN