This study presents Nuclear Resonance Vibrational Spectroscopy (NRVS) data on the five-coordinate (5C) ferrous heme nitrosyl complex [Fe(OEP)(NO)] (1, OEP2- = octaethylporphyrinato dianion) and the corresponding (NO)-N-15-O-18 labeled complex. The obtained spectra identify two isotope sensitive features at 522 and 388 cm(-1), which shift to 508 and 381 cm(-1), respectively, upon isotope labeling These features are assigned to the Fe-NO stretch p(Fe-NO) and the in-plane Fe-N-O bending mode delta(rp)(Fe-N-O), the latter has been unambiguously assigned for the first time for 1 The obtained NRVS data were simulated using our quantum chemistry centered normal coordinate analysis (QCC-NCA) Since complex 1 can potentially exist in 12 different conformations involving the FeNO and peripheral ethyl onentations, extended density functional theory (OFT) calculations and QCC-NCA simulations were performed to determine how these conformations affect the NRVS properties of [Fe(OEP)NO] These results show that the properties and force constants of the FeNO unit are hardly affected by the conformational changes involving the ethyl substituents On the other hand, the NRVS-active porphynn-based vibrations around 340-360, 300-320, and 250-270 cm-1 are sensitive to the conformational changes. The spectroscopic changes observed in these regions are due to selective mechanical couplings of one component of E-0-type (in ideal Do symmetry) porphyrin-based vibrations with the in-plane Fe-N-O bending mode This leads to the observed variations in Fe(OEP) core mode energies and NRVS intensities without affecting the properties of the FeNO unit The QCC-NCA simulated NRVS spectra of 1 show excellent agreement with experiment, and indicate that conformer F is likely present in the samples of this complex investigated here. The observed porphynn-based vibrations in the NRVS spectra of 1 are also assigned based on the QCC-NCA results The obtained force constants of the Fe NO and N-O bonds are 283-2.94 (based on the OFT functional applied) and about 12.15 mdyn/angstrom, respectively. The electronic structures of 5C ferrous heme-nitrosyls in different model complexes are then analyzed, and variations in their properties based on different porphyrin substituents are explained. Finally, the shortcomings of different DFT functionals in describing the axial FeNO subunit in heme-nitrosyls are elucidated