A nanoparticle polymer (NPP) is prepared from amino acids of keratin hydrolysate, obtained after enzymatic degradation. The NPP copolymer chain is made up of two types of monomers, viz an acrylic ester and an amino: acid derivative of keratin hydrolysate. NPP was synthesized using microemulsion polymerization method. The developed. NPR exhibits harrow particle size distribution with particle size of 60 nm. The NPP is a free flowing liquid With flow characteristics almost similar to water,. as Shown from its low relative viscosity (eta r) of 1.02 at 25 degrees C. The zeta potential of NPP at -30.2 my demonstrates anionic nature of the copolymer particle and this may be provided from amino acid derived monomer. The NPP possesses a high molecular weight copolymer in aqueous medium and carboxyl groups as an active, site for,interaction,. The dyeing process Carried out after the treatment of NPP enhanced dye uptake. From PM6 semi, empirical quantum mechanical level; the interaction of NPP With dye showed four reactive sites. The geometries of the reactive sites were found out The results showed that binding energies of site 2 and 3 were higher in comparison with those of 1 and 4. The kinetics and color Measurement study showed that dye uptake was enhanced by using NPP.