A lactobionic amide amphiphile was synthesized and used to functionalize multiwalled carbon nanotubes (MWNTs). The self-assembly of the amphiphile on the MWNTs was carried out in alcohol/water solutions. The functionalization of the MWNTs was investigated by means of ultraviolet, Raman, and X-ray photoelectron spectroscopies; X-ray diffraction; and high-resolution transmission electron microscopy. It was found that alcohols can promote the self-assembly of the amphiphile on MWNTs and regulate the amount of amphiphile assembled. Lysozyme was adsorbed onto fiinctionalized MWNTs. Circular dichroism (CD) spectra revealed that the conformation of lysozyme underwent no significant alteration upon adsorption.