Modulation of Cell Adhesion of Heparin-Based Hydrogel by Efficient Physisorption of Adhesive Proteins

Mihye Kim; Yang-Jung Kim; Kihak Gwon; Giyoong Tae

Macromolecular Research, Vol.20, No.3, 271-276, March, 2012

DOI10.1007/s13233-012-0058-6 Export Citation

Modulation of Cell Adhesion of Heparin-Based Hydrogel by Efficient Physisorption of Adhesive Proteins

Mihye Kim, Yang-Jung Kim, Kihak Gwon, and Giyoong Tae^†

School of Materials Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, 500-712, Korea

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Fibrinogen as well as collagen, cell adhesive proteins, were adsorbed efficiently onto a heparin-based hydrogel. The physisorption of these proteins was strong and stable so that the bound proteins were not easily released from the hydrogel at physiological condition. After the physisorption of fibrinogen or collagen type I onto the hydrogel, National Institute of Health/3-day transfer, inoculum 3×10^(5) cells (NIH/3T3) fibroblasts attached and spread well on the hydrogel, whereas the bare hydrogel or serum treated hydrogel showed poor cell attachment. Cell proliferation was also remarkably enhanced by the physisorption of these cell adhesive proteins, similar to that on tissue culture plate.

Keywords:heparin;hydrogel;fibrinogen;collagen;fibroblast;actin.

[References]

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Friedl P, Brocker EB, Cell. Mol. Life Sci., 57, 41 (2000)
Gumbiner BM, Cell., 84, 345 (1996)
Bacakova L, Filova E, Rypacek F, Svorcik V, Stary V, Physiol. Res., 53, S35 (2004)
Matsumura K, Hyon SH, Nakajima N, Peng C, Tsutsumi S, J. Biomed. Mater. Res., 50, 512 (2000)
Massia SP, Hubbell JA, J. Biomed. Mater. Res., 25, 223 (1991)
Kim KM, Herrera GA, Battarbee HD, Am. J.Pathol., 154, 843 (1999)
Chinn JA, in The Biomedical Engineering Handbook, CRC Press, Connecticut, 1597 (1995)
Healy KE, Rezania A, Stile RA, Ann. N.Y. Acad. Sci., 875, 24 (1999)
Tae G, Kim YJ, Choi WI, Kim M, Stayton PS, Hoffman AS, Biomacromolecules, 8(6), 1979 (2007)
Dudas J, Ramadori G, Knittel T, Neubauer K, Raddatz D, Egedy K, Kovalszky I, Biochem. J., 350, 245 (2000)
Lin RZ, Chang HY, Biotechnol. J., 3, 1171 (2008)
Schmidt KH, Ascencio F, Fransson LA, Kohler W, Wadstrom T, Zentralbl. Bakteriol., 279, 472 (1993)
Mosesson MW, J. Thromb. Haemost., 3, 1894 (2005)
Kim M, Shin Y, Hong BH, Kim YJ, Chun JS, Tae G, KimYH, Tissue Eng. Part C: Methods., 16, 1 (2010)
Il Choi W, Kim M, Tae G, Kim YH, Biomacromolecules, 9(6), 1698 (2008)
Vistica DT, Skehan P, Scudiero D, Monks A, Pittman A, Boyd MR, Cancer Res., 51, 2515 (1991)
Jaiswal M, Koul V, Dinda AK, Mohanty S, Jain KG, J. Biomed. Mater. Res. Part B: Appl. Biomater., 98B, 342 (2011)
Uchiyama S, Isobe K, Nagai S, Comp. Biochem. Physiol. B., 102, 343 (1992)
Odrljin TM, Shanioff JR, Lawrence SO, Sinpson-Haidaris PJ, Blood., 88, 2050 (1996)
Raut S, Gaffney PJ, Thromb. Res., 81, 503 (1996)
UI’ianov AM, Liapiana LA, Nauchnye Doki. Vyss. Shkoly Biol. Nauki., 117, 44 (1973)

[Referenced By]

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[1] Hunt TK, Hop H, Int. J. Low. Extrem. Wounds., 1, 68 (2002)

[2] Friedl P, Brocker EB, Cell. Mol. Life Sci., 57, 41 (2000)

[3] Gumbiner BM, Cell., 84, 345 (1996)

[4] Bacakova L, Filova E, Rypacek F, Svorcik V, Stary V, Physiol. Res., 53, S35 (2004)

[5] Matsumura K, Hyon SH, Nakajima N, Peng C, Tsutsumi S, J. Biomed. Mater. Res., 50, 512 (2000)

[6] Massia SP, Hubbell JA, J. Biomed. Mater. Res., 25, 223 (1991)

[7] Kim KM, Herrera GA, Battarbee HD, Am. J.Pathol., 154, 843 (1999)

[8] Chinn JA, in The Biomedical Engineering Handbook, CRC Press, Connecticut, 1597 (1995)

[9] Healy KE, Rezania A, Stile RA, Ann. N.Y. Acad. Sci., 875, 24 (1999)

[10] Tae G, Kim YJ, Choi WI, Kim M, Stayton PS, Hoffman AS, Biomacromolecules, 8(6), 1979 (2007)

[11] Dudas J, Ramadori G, Knittel T, Neubauer K, Raddatz D, Egedy K, Kovalszky I, Biochem. J., 350, 245 (2000)

[12] Lin RZ, Chang HY, Biotechnol. J., 3, 1171 (2008)

[13] Schmidt KH, Ascencio F, Fransson LA, Kohler W, Wadstrom T, Zentralbl. Bakteriol., 279, 472 (1993)

[14] Mosesson MW, J. Thromb. Haemost., 3, 1894 (2005)

[15] Kim M, Shin Y, Hong BH, Kim YJ, Chun JS, Tae G, KimYH, Tissue Eng. Part C: Methods., 16, 1 (2010)

[16] Il Choi W, Kim M, Tae G, Kim YH, Biomacromolecules, 9(6), 1698 (2008)

[17] Vistica DT, Skehan P, Scudiero D, Monks A, Pittman A, Boyd MR, Cancer Res., 51, 2515 (1991)

[18] Jaiswal M, Koul V, Dinda AK, Mohanty S, Jain KG, J. Biomed. Mater. Res. Part B: Appl. Biomater., 98B, 342 (2011)

[19] Uchiyama S, Isobe K, Nagai S, Comp. Biochem. Physiol. B., 102, 343 (1992)

[20] Odrljin TM, Shanioff JR, Lawrence SO, Sinpson-Haidaris PJ, Blood., 88, 2050 (1996)

[21] Raut S, Gaffney PJ, Thromb. Res., 81, 503 (1996)

[22] UI’ianov AM, Liapiana LA, Nauchnye Doki. Vyss. Shkoly Biol. Nauki., 117, 44 (1973)