Enhanced Gene Delivery by Palmitic Acid-Conjugated Low Molecular Weight Polyethylenimine

Duhwan Lee; Kaushik Singha; Juhee Park; Seongbong Jo; Won Jong Kim

Macromolecular Research, Vol.20, No.3, 244-249, March, 2012

DOI10.1007/s13233-012-0050-1 Export Citation

Enhanced Gene Delivery by Palmitic Acid-Conjugated Low Molecular Weight Polyethylenimine

Duhwan Lee, Kaushik Singha, Juhee Park, Seongbong Jo¹, and Won Jong Kim^†

Department of Chemistry, BK21 Program, Polymer Research Institute, Pohang University of Science and Technology, Gyeongbuk, 790-784, Korea
¹Department of Pharmaceutics, School of Pharmacy, The University of Mississippi, University, MS, 38677, USA

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Palmitic acid-conjugated low molecular weight polyethylenimine (PEI-g-PEG-PA) was successfully synthesized to develop an efficient non-viral gene carrier. The judicious integration of hydrophobic palmitic acid and polyethylenimine via hydrophilic polyethylene glycol (PEG) facilitated the formation of nano-sized complex (nanoplex) with plasmid DNA (pDNA) which provided the protection of pDNA against the serumic enzymatic degradation. Furthermore, the delivery system demonstrated enhanced gene transfection efficiency in comparison to unmodified low molecular weight PEI without inducing any significant cytotoxicity.

Keywords:gene delivery;polyethylenimine;nanoplex;non-viral;palmitic acid.

[References]

Muzyczka N, Curr. Top. Microbiol. Immunol., 158, 97 (1992)
Varmus H, Science., 240, 1427 (1998)
Boussif O, Lezoualc’h F, Zanta MA, Mergny MD, Scherman D, Demeneix B, Behr JP, Proc. Natl. Acad.Sci. USA., 92, 7297 (1995)
Godbey WT, Wu KK, Mikos AG, J. Biomed. Mater.Res., 45, 268 (1999)
Kim WJ, Kim SW, Macromol. Res., 15(2), 100 (2007)
Namgung R, Kim J, Singha K, Kim CH, Kim WJ, Mol. Pharm., 6, 1826 (2009)
Hunter AC, Moghimi SM, Drug Discov. Today., 7, 998 (2002)
Akiyama Y, Harada A, Nagasaki Y, Kataoka K, Macromolecules, 33(16), 5841 (2000)
Vinogradov SV, Bronich TK, Kabanov AV, Bioconjug.Chem., 9, 805 (1998)
Ahn CH, Chae SY, Bae YH, Kim SW, J. Control.Release., 80, 273 (2002)
Son S, Singha K, Kim WJ, Biomaterials., 31, 6344 (2010)
Son S, Hwang DW, Singha K, Jeong JH, Park TG, Lee DS, Kim WJ, J. Control. Release., 155, 18 (2011)
Han S, Mahato RI, Kim SW, Bioconjug. Chem., 12, 337 (2001)
Yockman JW, Maheshwari A, Han SO, Kim SW, J.Control. Release., 87, 177 (2003)
Kim WJ, Chang CW, Lee M, Kim SW, J. Control. Release., 118, 357 (2007)
Zhu J, Tang A, Law LP, Feng M, Ho KM, Lee DKL, Harris FW, Li P, Bioconjug. Chem., 16, 139 (2005)
Shuai XT, Merdan T, Unger F, Wittmar M, Kissel T, Macromolecules, 36(15), 5751 (2003)
Brownlie A, Uchegbu IF, Schatzlein AG, Int. J. Pharm., 274, 41 (2004)
Read ML, Dash PR, Clark A, Howard KA, Oupickya D, Toncheva V, Alpar HO, Schacht EH, Ulbrich K, Seymour LW, Eur. J. Pharm. Sci., 10, 169 (2000)
Kim WJ, Yockman JW, Lee M, Jeong JH, Kim YH, Kim SW, J. Control. Release., 106, 224 (2005)
Chollet P, Favrot MC, Hurbin A, Coll JL, J. Gene Med., 4, 84 (2002)
Fischer D, Li Y, Ahlemeyer B, Krieglstein J, Kissel T, Biomaterials., 24, 1121 (2003)
Bennett CF, Chiang MY, Chan H, Shoemaker JE, Mirabelli CK, Mol. Pharmacol., 41, 1023 (1992)
Zuidam NJ, Posthuma G, de Vries ETJ, Crommelin DJA, Hennink WE, Storm G, J. Drug Target., 8, 51 (2000)
Mishra S, Webster P, Davis ME, Eur. J. Cell Biol., 83, 97 (2004)
Ogris M, Brunner S, Schuller S, Kircheis R, Wagner E, Gene Ther., 6, 596 (1999)
Needham D, Mcintosh TJ, Lasic DD, Biochim. Biophys. Acta., 1108, 40 (1992)
Collard WT, Yang Y, Kwok KY, Park Y, Rice KG, J. Pharm. Sci., 89, 499 (2000)

[Referenced By]

[1] Muzyczka N, Curr. Top. Microbiol. Immunol., 158, 97 (1992)

[2] Varmus H, Science., 240, 1427 (1998)

[3] Boussif O, Lezoualc’h F, Zanta MA, Mergny MD, Scherman D, Demeneix B, Behr JP, Proc. Natl. Acad.Sci. USA., 92, 7297 (1995)

[4] Godbey WT, Wu KK, Mikos AG, J. Biomed. Mater.Res., 45, 268 (1999)

[5] Kim WJ, Kim SW, Macromol. Res., 15(2), 100 (2007)

[6] Namgung R, Kim J, Singha K, Kim CH, Kim WJ, Mol. Pharm., 6, 1826 (2009)

[7] Hunter AC, Moghimi SM, Drug Discov. Today., 7, 998 (2002)

[8] Akiyama Y, Harada A, Nagasaki Y, Kataoka K, Macromolecules, 33(16), 5841 (2000)

[9] Vinogradov SV, Bronich TK, Kabanov AV, Bioconjug.Chem., 9, 805 (1998)

[10] Ahn CH, Chae SY, Bae YH, Kim SW, J. Control.Release., 80, 273 (2002)

[11] Son S, Singha K, Kim WJ, Biomaterials., 31, 6344 (2010)

[12] Son S, Hwang DW, Singha K, Jeong JH, Park TG, Lee DS, Kim WJ, J. Control. Release., 155, 18 (2011)

[13] Han S, Mahato RI, Kim SW, Bioconjug. Chem., 12, 337 (2001)

[14] Yockman JW, Maheshwari A, Han SO, Kim SW, J.Control. Release., 87, 177 (2003)

[15] Kim WJ, Chang CW, Lee M, Kim SW, J. Control. Release., 118, 357 (2007)

[16] Zhu J, Tang A, Law LP, Feng M, Ho KM, Lee DKL, Harris FW, Li P, Bioconjug. Chem., 16, 139 (2005)

[17] Shuai XT, Merdan T, Unger F, Wittmar M, Kissel T, Macromolecules, 36(15), 5751 (2003)

[18] Brownlie A, Uchegbu IF, Schatzlein AG, Int. J. Pharm., 274, 41 (2004)

[19] Read ML, Dash PR, Clark A, Howard KA, Oupickya D, Toncheva V, Alpar HO, Schacht EH, Ulbrich K, Seymour LW, Eur. J. Pharm. Sci., 10, 169 (2000)

[20] Kim WJ, Yockman JW, Lee M, Jeong JH, Kim YH, Kim SW, J. Control. Release., 106, 224 (2005)

[21] Chollet P, Favrot MC, Hurbin A, Coll JL, J. Gene Med., 4, 84 (2002)

[22] Fischer D, Li Y, Ahlemeyer B, Krieglstein J, Kissel T, Biomaterials., 24, 1121 (2003)

[23] Bennett CF, Chiang MY, Chan H, Shoemaker JE, Mirabelli CK, Mol. Pharmacol., 41, 1023 (1992)

[24] Zuidam NJ, Posthuma G, de Vries ETJ, Crommelin DJA, Hennink WE, Storm G, J. Drug Target., 8, 51 (2000)

[25] Mishra S, Webster P, Davis ME, Eur. J. Cell Biol., 83, 97 (2004)

[26] Ogris M, Brunner S, Schuller S, Kircheis R, Wagner E, Gene Ther., 6, 596 (1999)

[27] Needham D, Mcintosh TJ, Lasic DD, Biochim. Biophys. Acta., 1108, 40 (1992)

[28] Collard WT, Yang Y, Kwok KY, Park Y, Rice KG, J. Pharm. Sci., 89, 499 (2000)