Characterization of purified phospholipids from krill (Euphausia superba) residues deoiled by supercritical carbon dioxide

Abdelkader Ali-Nehari; Byung-Soo Chun

Korean Journal of Chemical Engineering, Vol.29, No.7, 918-924, July, 2012

DOI10.1007/s11814-011-0273-4 Export Citation

Characterization of purified phospholipids from krill (Euphausia superba) residues deoiled by supercritical carbon dioxide

Abdelkader Ali-Nehari, and Byung-Soo Chun^{1, †}

International Program of Fisheries Sciences, Faculty of Fisheries Sciences, Pukyong National University, Busan 608-737, Korea
¹Department of Food Science and Technology, Pukyong National University, Busan 608-737, Korea

E-mail:

Purification of phospholipids (PL) from the Antarctic krill (Euphausia superba) using a two-step extraction process has been investigated. Using supercritical carbon dioxide (SC-CO2) extraction with optimal extractions conditions of 45℃ , 25MPa, and CO2 flow rate of 22 g/min, most of the neutral lipids were extracted. PC, PE and PI were then extracted in a second step conducted with modified existing method using ethanol, hexane and acetone as solvents. The major PL of krill residues was quantified by high performance liquid chromatography (HPLC-ELSD). The fatty acid compositions of total PL, PC, PE and PI were analyzed by gas chromatography (GC). A significant amount of polyunsaturated fatty acids (PUFA) was present in both total and PLs fractions. The purified PLs were characterized by their acid value, peroxide value, and the oxidative stability. The purity of PL ranged between 93 and 97% and was evaluated by spectrophotometry.

Keywords:Krill;Phospholipids Purification;Supercritical Carbon Dioxide;Oxidative Stability

[References]

Martin A, Phytotherapie., HS, 6 (2007)
Peterson BL, Cummings BS, Biomed. Chromatography., 20, 227 (2005)
Budavari S, (Eds.) The Merck Index, Volume 11, Merck and Co.,Rahway, New Jersey, USA (1989)
Caboni MF, Menotta S, Lercker G, J. Am. Oil Chem. Soc., 73, 1561 (1996)
Boselli E, Pacetti D, Curzi F, Frega NG, J. Meat Sci., 78, 305 (2008)
Sihvonen M, Jarvenpaa E, Hietaniemi V, Huopalahti R, Trends Food Sci. Technol., 10, 217 (1999)
Hong SA, Kim J, Kim JD, Kang JW, Lee YW, Sep. Sci. Technol., 45(8), 1139 (2010)
Yamaguchi K, Murakami M, Nakano H, Konosu S, Kokura T, Yamamoto H, Kosaka M, Hata K, J. Agric. Food Chem., 34, 904 (1986)
Yukihisa T, Ikuko S, Takeshi O, J. Oleo. Sci., 53, 417 (2004)
Montanari L, King JW, List GR, Rennick KA, J. Food Sci., 61, 1230 (1996)
Prosise WE, American Oil Chemists’ Society, Champaign IL, 163 (1985)
Palacios LE, Wang T, J. AOCS., 82, 571 (2005)
Stewart JCM, Anal. Biochem., 104, 10 (1980)
Letter WS, J. Liquid Chromatography., 15, 253 (1992)
AOCS, in Official methods and recommended practices of AOCS (4th Ed.), Firestone DE Ed., Illinois, AOCS Press (2001)
Bernardez M, Pastoriza L, Sampedro G, Herrera JJ, Cabo ML, J. Agric. Food Chem., 53, 1903 (2005)
Lowry RR, Tinsley IJ, J. Am. Oil Chem. Soc., 7, 470 (1976)
Fricke H, Gercken G, Schreiber W, Oehlenschlager J, J. Lipids., 19, 821 (1984)
AOCS, American Oil Chemists' Society, Champaign, IL (1997)
Mitsuda H, Yasumoto K, Iwami K, Eiyo to Shokuryo., 19, 210 (1966)
Ottolenghi A, Arch. Biochem. Biophys., 79, 355 (1959)
Morita A, Kajimoto O, J. Phys. Chem., 94, 6420 (1990)
de Azevedo ABA, Kieckbush TG, Tashima AK, Mohamed RS, Mazzafera P, de Melo SABV, J. Supercrit. Fluids, 44(2), 186 (2008)
Macias-Sanchez MD, Mantell C, Rodriguez M, de la Ossa EM, Lubian LM, Montero O, J. Supercrit. Fluids, 39(3), 323 (2007)
Bottino NR, Comp. Biochem. Phys., 50B, 479 (1975)
Kusumuto N, Ando Y, Matsukura R, Mukai T, J. Oleo Sci., 53, 45 (2004)
Gordeev KY, Filarin VN, Bondarenko SV, Kirpichenok MA, Gordeeva NA, Grandberg II, Batrakov SG, Chemistry of Natural Compounds., 26, 143 (2004)
Ackman RG, Eaton CA, Sipos JC, Hooper SN, Castell JD, J. Fish. Res. Board Can., 27, 513 (1970)
Sargent JR, Falk-Petersen S, Mar. Biol., 62, 131 (1981)
Saether O, Ellingsen TE, Mohr V, J. Lipid Res., 27, 274 (1986)
Rubio-Rodriguez N, de Diego SM, Beltran S, Jaime I, Sanz MT, Rovira J, J. Supercrit. Fluids, 47(2), 215 (2008)
Yanagimoto M, Shibasaki S, Suguira H, Shimazaki E, Umeda K, Kimura S, Nippon Shokuhin Kogyo Gakkai-Shi., 26, 151 (1979)
Chapelle S, Biochem. Syst. Ecol., 5, 241 (1977)
Chapelle, Comp. Biochem. Phys., 84B, 423 (1986)
Lee JH, Fujimoto K, Kaneda T, Bulletin of the Japanese Society of Scientific Fisheries., 47, 881 (1981)
Shinichi T, Kumi M, Masahisa N, Mizuho M, Satoshi H, Comparative Biochemistry and Physiology., B 136, 317 (2003)
Gogolewski M, Nogala-Kalucka M, Szeliga M, European J. Lipid Sci. Technol., 102, 618 (2000)
Belhaj N, Tehrany AE, Linder M, Process Biochem., 45, 187 (2010)

[Referenced By]

[1] Martin A, Phytotherapie., HS, 6 (2007)

[2] Peterson BL, Cummings BS, Biomed. Chromatography., 20, 227 (2005)

[3] Budavari S, (Eds.) The Merck Index, Volume 11, Merck and Co.,Rahway, New Jersey, USA (1989)

[4] Caboni MF, Menotta S, Lercker G, J. Am. Oil Chem. Soc., 73, 1561 (1996)

[5] Boselli E, Pacetti D, Curzi F, Frega NG, J. Meat Sci., 78, 305 (2008)

[6] Sihvonen M, Jarvenpaa E, Hietaniemi V, Huopalahti R, Trends Food Sci. Technol., 10, 217 (1999)

[7] Hong SA, Kim J, Kim JD, Kang JW, Lee YW, Sep. Sci. Technol., 45(8), 1139 (2010)

[8] Yamaguchi K, Murakami M, Nakano H, Konosu S, Kokura T, Yamamoto H, Kosaka M, Hata K, J. Agric. Food Chem., 34, 904 (1986)

[9] Yukihisa T, Ikuko S, Takeshi O, J. Oleo. Sci., 53, 417 (2004)

[10] Montanari L, King JW, List GR, Rennick KA, J. Food Sci., 61, 1230 (1996)

[11] Prosise WE, American Oil Chemists’ Society, Champaign IL, 163 (1985)

[12] Palacios LE, Wang T, J. AOCS., 82, 571 (2005)

[13] Stewart JCM, Anal. Biochem., 104, 10 (1980)

[14] Letter WS, J. Liquid Chromatography., 15, 253 (1992)

[15] AOCS, in Official methods and recommended practices of AOCS (4th Ed.), Firestone DE Ed., Illinois, AOCS Press (2001)

[16] Bernardez M, Pastoriza L, Sampedro G, Herrera JJ, Cabo ML, J. Agric. Food Chem., 53, 1903 (2005)

[17] Lowry RR, Tinsley IJ, J. Am. Oil Chem. Soc., 7, 470 (1976)

[18] Fricke H, Gercken G, Schreiber W, Oehlenschlager J, J. Lipids., 19, 821 (1984)

[19] AOCS, American Oil Chemists' Society, Champaign, IL (1997)

[20] Mitsuda H, Yasumoto K, Iwami K, Eiyo to Shokuryo., 19, 210 (1966)

[21] Ottolenghi A, Arch. Biochem. Biophys., 79, 355 (1959)

[22] Morita A, Kajimoto O, J. Phys. Chem., 94, 6420 (1990)

[23] de Azevedo ABA, Kieckbush TG, Tashima AK, Mohamed RS, Mazzafera P, de Melo SABV, J. Supercrit. Fluids, 44(2), 186 (2008)

[24] Macias-Sanchez MD, Mantell C, Rodriguez M, de la Ossa EM, Lubian LM, Montero O, J. Supercrit. Fluids, 39(3), 323 (2007)

[25] Bottino NR, Comp. Biochem. Phys., 50B, 479 (1975)

[26] Kusumuto N, Ando Y, Matsukura R, Mukai T, J. Oleo Sci., 53, 45 (2004)

[27] Gordeev KY, Filarin VN, Bondarenko SV, Kirpichenok MA, Gordeeva NA, Grandberg II, Batrakov SG, Chemistry of Natural Compounds., 26, 143 (2004)

[28] Ackman RG, Eaton CA, Sipos JC, Hooper SN, Castell JD, J. Fish. Res. Board Can., 27, 513 (1970)

[29] Sargent JR, Falk-Petersen S, Mar. Biol., 62, 131 (1981)

[30] Saether O, Ellingsen TE, Mohr V, J. Lipid Res., 27, 274 (1986)

[31] Rubio-Rodriguez N, de Diego SM, Beltran S, Jaime I, Sanz MT, Rovira J, J. Supercrit. Fluids, 47(2), 215 (2008)

[32] Yanagimoto M, Shibasaki S, Suguira H, Shimazaki E, Umeda K, Kimura S, Nippon Shokuhin Kogyo Gakkai-Shi., 26, 151 (1979)

[33] Chapelle S, Biochem. Syst. Ecol., 5, 241 (1977)

[34] Chapelle, Comp. Biochem. Phys., 84B, 423 (1986)

[35] Lee JH, Fujimoto K, Kaneda T, Bulletin of the Japanese Society of Scientific Fisheries., 47, 881 (1981)

[36] Shinichi T, Kumi M, Masahisa N, Mizuho M, Satoshi H, Comparative Biochemistry and Physiology., B 136, 317 (2003)

[37] Gogolewski M, Nogala-Kalucka M, Szeliga M, European J. Lipid Sci. Technol., 102, 618 (2000)

[38] Belhaj N, Tehrany AE, Linder M, Process Biochem., 45, 187 (2010)