Alternative fuels for the transport sector are gaining growing attention as a means against fossil fuel dependence and towards greener forms of energy. At the same time, however, they are surrounded with doubts concerning sustainability of their production. This work presents the basic framework for a decision support tool to evaluate biofuel production pathways, with the purpose of providing the decision maker with a structured methodology that will lead him to the final decision. The tool integrates the most important aspects along the entire value chain (i.e. from biomass production to biofuel end-use), namely the technical, economic, environmental and social aspect. The tool consists of a computational part, which can be combined with the personal preferences of the user. The analysis provides a score for the respective pathway that can be used to rank different options and select among them the optimal solution. The functionality of the tool has been tested for the case of biodiesel from rapeseed in Germany. (C) 2011 Elsevier Ltd. All rights reserved.