In earlier work [1]. NIST developed a climate suitability analysis method to evaluate the potential of a given location for direct ventilative cooling and nighttime ventilative cooling. The direct ventilative cooling may be provided by either a natural ventilation system or a fan-powered economizer system. The climate suitability analysis is based on a general single-zone thermal model of a building configured to make optimal use of direct and/or nighttime ventilative cooling. This paper describes a new tool implementing this climate suitability methodology and its capability to consider an adaptive thermal comfort option and presents results from its application to analyze a variety of U.S. climates. The adaptive thermal comfort option has the potential to substantially increase the effectiveness of natural ventilation cooling for many U.S. cities. However, this impact is very dependent on the acceptable humidity range. If a dewpoint limit is used, the increase is significant for a dry climate such as Phoenix but much smaller for humid climates such as Miami. While ASHRAE Standard 55 does not impose a limit on humidity when using the adaptive thermal comfort option, the necessity of limiting humidity for other reasons needs to be considered. Published by Elsevier B.V.