Rapid and direct online preconcentration followed by CE with capacitively coupled contactless conductivity detection (CE-(CD)-D-4) is evaluated as a new approach for the determination of glyphosate, glufosinate (GLUF), and aminophosphonic acid (AMPA) in drinking water. Two online preconcentration techniques, namely large volume sample stacking without polarity switching and field-enhanced sample injection, coupled with CE-(CD)-D-4 were successfully developed and optimized. Under optimized conditions, LODs in the range of 0.01-0.1 mu M (1.7-11.1 mu g/L) and sensitivity enhancements of 48- to 53-fold were achieved with the large volume sample stacking-CE-(CD)-D-4 method. By performing the field-enhanced sample injection-CE-(CD)-D-4 procedure, excellent LODs down to 0.0005-0.02 mu M (0.1-2.2 mu g/L) as well as sensitivity enhancements of up to 245- to 1002-fold were obtained. Both techniques showed satisfactory reproducibility with RSDs of peak height of better than 10%. The newly established approaches were successfully applied to the analysis of glyphosate, glufosinate, and aminophosphonic acid in spiked tap drinking water.