The continued publication from year to year of new MEKC formulations for the analysis of amino acids provides evidence that both CZE and MEKC still have a great power of attraction in this area. The present review intends to cover the literature on MEKC of amino acids from 2007 until present: it has been planned to follow the same format of our previous review (Flectrophoresis 2008, 29, 224-236) representing its ideal continuation. In addition to methodological developments, the more recent practical applications of MEKC procedures for the determination of amino acids in different matrices will also be described here as an evidence, once again, of the suitability of this technique on samples of different origin.