Ovicidal activities of supercritical CO2 and N2O on Ascaris suum eggs

Sungmin Mun; Youn-Woo Lee; Jeyong Yoon

Journal of Industrial and Engineering Chemistry, Vol.18, No.1, 504-508, January, 2012

DOI10.1016/j.jiec.2011.11.032 Export Citation

Ovicidal activities of supercritical CO2 and N2O on Ascaris suum eggs

Sungmin Mun^{1, 2}, Youn-Woo Lee², and Jeyong Yoon^{1, 2, †}

¹World Class University (WCU) Program of Chemical Convergence for Energy & Environment (C2E2), College of Engineering, Seoul National University, Daehak-dong, Gwanak-gu, Seoul 151-742, Republic of Korea
²School of Chemical and Biological Engineering, College of Engineering, Seoul National University, Daehak-dong, Gwanak-gu, Seoul 151-742, Republic of Korea

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This study examined whether supercritical CO2 and N2O fluids are effective in inactivating Ascaris suum eggs, which were chosen as a model for parasite eggs. The treatments were carried out in a multibatch apparatus, in which, the eggs could be placed atop a solid surface (non-immersed condition) or in aqueous solution (immersed condition). Various CO2 and N2O phases-including gas (6 MPa, 30 ℃), liquid (8 MPa, 30 ℃), subcritical (10 MPa, 30 ℃), and supercritical (10 MPa, 37 ℃).were tested with exposure times ranging from 1 to 20 min. Supercritical CO2 and N2O both showed a similar, strong ovicidal effect, requiring only 1 min in non-immersed conditions and 5 min in water-immersed conditions to achieve a 2.4-log inactivation. Subcritical CO2 and N2O showed a weaker ovicidal effect. The effect was significantly reduced for the gas and liquid phases, compared with the supercritical phases. This study reports that supercritical CO2 and N2O can be effectively employed as a non-thermal treatment technique to control parasite egg contamination in fecal matter and food.

Keywords:Supercritical CO2;Supercritical N2O;Inactivation;Ascaris suum eggs

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[1] Crompton DWT, Adv. Parasitol., 48, 285 (2001)

[2] Dentinger CM, Bower WA, Nainan OV, Cotter SM, Myers G, Dubusky LM, Fowler S, Salehi ED, Bell BP, J. Infect. Dis., 183, 1273 (2001)

[3] Ho AY, Lopez AS, Eberhart MG, Levenon R, Finkel BS, Silva AJ, Roberts JM, Orlandi PA, Johnson CC, Herwaldt BL, Infect. Dis., 8, 783 (2002)

[4] Isaacs S, Aramini J, Ciebin B, Farrar JA, Ahmed R, Middleton D, Chandran AU, Harris LJ, Howes M, Chan E, Pichette AS, Campbell K, Gupta A, Lior LY, Pearce M, Clark C, Rodgers F, Jamieson F, Trophy I, Ellis A, J. Food Prot., 68, 191 (2005)

[5] Capizzi-Banas S, Deloge M, Remy M, Schwartzbrod J, Water Res., 38, 3251 (2004)

[6] Johnson PW, Dixon R, Ross AD, Int. J. Parasitol., 28, 627 (1998)

[7] Habluetzel A, Traldi G, Ruggieri S, Attili AR, Scuppa P, Marchetti R, Menghini G, Esposito F, Vet. Parasitol., 113, 243 (2003)

[8] O¨ zkayhan MA, J. Helminthol., 80, 15 (2006)

[9] Mun S, Cho SH, Kim TS, Oh BT, Yoon J, Chemosphere., 77, 285 (2009)

[10] Tharaldsen J, Helle O, Acta Agric. Scand. Sect. A: Anim. Sci., 39, 381 (1989)

[11] Pecson BM, Nelson KL, Environ. Sci. Technol., 39, 7909 (2005)

[12] Rosypal AC, Bowman DD, Holliman D, Flick GJ, Lindsay DS, Vet. Parasitol., 145, 86 (2007)

[13] Kim SR, Rhee MS, Kim BC, Lee H, Kim KH, J. Microbiol. Methods., 70, 132 (2007)

[14] Dillow AK, Dehghani F, Hrkach JS, Foster NR, Langer R, Proc. Natl. Acad. Sci.U.S.A., 96, 10344 (1999)

[15] Erkmen O, Int. J. Food Microbiol., 17, 133 (2001)

[16] Garcia-Gonzalez L, Geeraerd AH, Spilimbergo S, Elst K, Ginneken LV, Debevere J, Impe, Devlieghere F, Int. J. Food Microbiol., 117, 1 (2007)

[17] Spilimbergo S, Mantoan D, Dalser A, J. Supercrit. Fluids, 40(3), 485 (2007)

[18] Gasperi F, Aprea E, Biasioli F, Carlin S, Endrizzi I, Pirretti G, Spilimbergo S, Food Chem., 115, 129 (2009)

[19] Mun S, Hahn J, Lee Y, Yoon J, Int. J. Food Microbiol., 144, 372 (2011)

[20] Nguyen-Phan TD, Pham HD, Kim S, Oh ES, Kim EJ, Shin EW, J. Ind. Eng. Chem., 16(5), 823 (2010)

[21] Brownell SA, Nelson KL, Appl. Environ. Microbiol., 72, 2178 (2006)

[22] Oksanen A, Eriksen L, Roepstorff A, Ilsøe B, Nansen P, Lind P, Acta Vet. Scand., 31, 393 (1990)

[23] Bowman DD, Little MD, Reimers RS, Water Res., 37, 2063 (2003)

[24] Mun S, Jeong J, Kim J, Lee Y, Yoon J, Biofouling., 25, 473 (2009)

[25] King MB, Mubarak A, Kim JD, Bott TR, J. Supercrit. Fluids., 5, 296 (1992)

[26] Coan CR, King AD, J. Am. Chem. Soc., 93, 1857 (1971)

[27] Garcia-Gonzalez L, Geeraerd AH, Mast J, Briers Y, Elst K, Ginneken LV, Impe JFV, Devlieghere F, Food Microbiol., 27, 541 (2010)

[28] Hong S, Pyun Y, Int. J. Food Microbiol., 63, 19 (2001)

[29] Wharton D, Parasitology., 81, 447 (1980)

[30] Orta de Velasquez MT, Martinez JL, Monje-Ramirez I, Rojas-Valencia MN, Ozone-Sci. Eng.