화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.20, No.1, 93-100, January, 2012
DOI10.1007/s13233-012-0005-6  Export Citation
Characterization and Hepatocytes Adhesion of Galactosylated Poly(D,L-lactic-co-glycolic acid) Surface
Lee Choi1, 2, Soon Jong Kwak3, Su Jung You2, Heung Jae Chun1, 2, †, Hong Lim Kim4, Young Bock Shim5, Moon Suk Kim6, and Ki Dong Park6
  • 1Department of Biomedical Sciences, Catholic University, Seoul, 137-701, Korea
  • 2Institute of Cell & Tissue Engineering, Catholic University, Seoul, 137-701, Korea
  • 3Polymer Hybrid Research Center, Korea Institute of Science and Technology, Seoul, 136-791, Korea
  • 4Integrative Research Support Center, College of Medicine, Catholic University, Seoul, 137-701, Korea
  • 5Research Institute of Biomedical Engineering, Korea Bone Bank Co. Ltd., Seoul, 153-782, Korea
  • 6Department of Molecular Science and Technology, Ajou University, Gyeonggi, 443-759, Korea
E-mail:
The present study demonstrated that covalently galactosylated poly(D,L-lactic-co-glycolic acid) (PLGA) surface encourages hepatocyte adhesion and growth to form a dense cell network. Galactosylation of the PLGA surface was accomplished by grafting allylamine (AA) using inductively coupled plasma-assisted chemical vapor deposition (ICP-CVD) and conjugating lactobionic acid (LA) with AA via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide (EDC/NHS) activation. The modified surface was characterized by Fourier transform infrared spectroscopy in the attenuated total reflectance, electron spectroscopy of chemical analysis, atomic force microscopy, and contact angle measurement. For evaluation of cell affinity in vitro, primary rat hepatocytes were prepared and seeded onto the modified PLGA surfaces. The galactosylated PLGA surface showed more pronounced hepatocyte adhesion and growth compared to those on the control PLGA surface. The hepatocytes seeded on galactosylated substrates exhibited a radial migration with filopodial growth to form multicellular aggregates, whereas those on control PLGA showed slowly adhered rounded shapes. Moreover, galactosylation increased metabolic hepatocyte activities such as albumin secretion and urea synthesis.
Keywords:poly(D,L-lactic-co-glycolic acid) (PLGA);allylamine;inductively coupled plasma-assisted chemical vapor deposition (ICP-CVD);galactose ligand;hepatocyte;multicellular aggregates.
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