The Trp-His-Thr-Leu-Pro-Asn-Ala sequence, which preferentially recognizes cis-form azobenzene groups of synthetic polymers, was fused to the N-terminus of green fluorescent protein. Quartz crystal microbalance measurements were used to investigate its absorption behavior on the polymer film surfaces. The results indicated that the peptide succeeded in immobilizing the fused protein onto the film surfaces containing cis-form azobenzene groups.