In our previous work, we have investigated the adsorption selectivity of CH4/H-2 in three pairs of isoreticular metal-organic frameworks (IRMOFs) with and without interpenetration to study the effect of interpenetration on gas mixture separation through Monte Carlo simulation. In addition, the self-diffusivities and the diffusion mechanism of single H-2 and CH4 in these MOFs were examined by molecular dynamics simulations. In this work, we extend our previous work to mixed-ligand MOFs to investigate the effects of interpenetration as well as mixed-ligand on both equilibrium-based and kinetic-based gas mixture separation. We found that methane adsorption selectivity is much enhanced in the selected mixed-ligand interpenetrated MOFs compared with their non-interpenetrated counterparts, similar to what we found before for IRMOFs with single-ligand. At room temperature and atmospheric pressure, molecular-level segregation was observed in the mixed-ligand MOFs, and the extent of the effects of interpenetration is comparable for single-ligand and mixed-ligand MOFs. In addition, we found that the diffusion selectivity in the interpenetrated MOFs is similar to the one in their non-interpenetrated counterparts, while the permeation selectivity in the former is much higher than that in the latter, which corroborates our expectation that interpenetration is a good strategy to improve the overall performance of a material as a membrane in separation applications based only on the single component diffusion results. Furthermore, the CH4 permeability of the selected MOF membrane was also evaluated. (C) 2011 Elsevier Ltd. All rights reserved.