Biotechnology Letters, Vol.33, No.2, 333-338, 2011
Specific inhibition of the halogenase for radicicol biosynthesis by bromide at the transcriptional level in Pochonia chlamydosporia
Pochonia chlamydosporia produces radicicol (1), a potent antifungal and anticancer product. NaBr, but not NaF, NaCl or NaI, inhibited the biosynthesis of 1 in P. chlamydosporia in a dose-dependent manner, accompanied by the formation chlorine-lacking monocillins II-V (2-5), indicating that the dedicated halogenase, Rdc2 had been inhibited. RT-PCR analysis confirmed that transcription of rdc2 was selectively inhibited by Br-, whereas the putative P450 epoxidase gene, rdc4, was not affected.