Bovine lactoferrin (BLF) enhanced production of hyaluronan in normal human dermal fibroblasts. The elevation of hyaluronan was accompanied by elevation of HAS2 (hyaluronan synthase 2) mRNA transcription and HAS2 protein expression. The promoting effect of BLF was not observed for HAS1. In addition, COL1A1 transcription and collagen synthesis were enhanced by BLF. These observations suggest that BLF promotes wound healing by increasing hyaluronan and type-I collagen synthesis.