Decellularized PLGA-Based Scaffolds and Their Osteogenic Potential with Bone Marrow Stromal Cells

Yu Jin Hong; Soon Eon Bae; Sun Hee Do; Ik Hwan Kim; Dong Keun Han; Kwideok Park

Macromolecular Research, Vol.19, No.10, 1090-1096, October, 2011

DOI10.1007/s13233-011-1004-8 Export Citation

Decellularized PLGA-Based Scaffolds and Their Osteogenic Potential with Bone Marrow Stromal Cells

Yu Jin Hong^{1, 2}, Soon Eon Bae^{1, 3}, Sun Hee Do⁴, Ik Hwan Kim², Dong Keun Han^{1, 3}, and Kwideok Park^{1, 3, †}

¹Center for Biomaterials, Korea Institute of Science and Technolog, Seoul, 130-650, Korea
²Department of Biological Science, Korea University, Seoul, 136-701, Korea
³, Korea
⁴College of Veterinary Medicine, Konkuk University, Seoul, 143-701, Korea

E-mail:

A cell-derived extracellular matrix (ECM) was naturally obtained and its effect on the induction of osteogenesis of bone marrow stromal cells (BMSCs) was investigated. Once porous composite scaffolds made of poly(Llactic-co-glycolic acid) (PLGA), hydroxyapatite (HA), and β-tricalcium phosphate (β-TCP) were fabricated, these scaffolds were seeded with fibroblasts or preosteoblasts and cultured in vitro. They were then subjected to decellularization, resulting in fibroblasts-decellularized scaffolds (FDS) or preosteoblasts-decellularized scaffolds (PDS). Both fibronectin and type I collagen were clearly detected from the immunofluorescent staining of FDS and PDS, respectively. When the rabbit BMSCs-loaded scaffolds were cultured in the osteogenic medium for 4 weeks, the osteogenic potential of FDS and PDS was much greater than the PLGA/HA/β-TCP (control), as identified by histological staining and the alkaline phosphatase (ALP) activity. Meanwhile, when BMSC-seeded FDS was implanted subcutaneously into the nude mice, the results also indicated more upregulated osteogenic differentiation of BMSCs in vivo compared to the control. This study suggests that the microenvironment created by cell-derived ECM can provide a favorable template in prompting the osteogenesis of BMSCs.

Keywords:bone marrow stromal cells;osteogenic differentiation;extracellular matrix;scaffold;decellularization.

[References]

Discher DE, Mooney DJ, Zandstra PW, Science, 324, 1673 (2009)
Juliano RL, Haskill S, J. Cell Biol., 120, 577 (1993)
Badylak SF, in Encyclopedia of Biomaterials and Biomedical Engineering, Wnek GE, Bowlin GL, Eds., Marcel Dekker Inc., New York, 2004, p 561.
Ott HC, Matthiesen TS, Goh SK, Black LD, Kren SM, Netoff TI, Taylor DA, Nat. Med., 14, 213 (2008)
Iop L, Renier V, Naso F, Piccoli M, Bonetti A, Gandaglia A, Pozzobon M, Ortolani F, Marchini M, Spina M, Coppip D, Sartore S, Gerosa G, Biomaterials, 30, 4104 (2009)
Park SN, Kim JK, Suh H, Biomaterials, 25, 3689 (2004)
Serban MA, Liu Y, Prestwich GD, Acta Biomater., 4, 67 (2008)
Bhati RS, Mukherjee DP, McCarthy KJ, Rogers SH, Smith DF, Shalaby SW, J. Biomed. Mater. Res., 56, 74 (2001)
Zhang H, Tasnim F, Ying YJ, Zink D, Biomaterials, 30, 2899 (2009)
Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR, Science, 284, 143 (1999)
Pelham RJ, Wang Y, Proc. Natl. Acad. Sci., 94, 13661 (1997)
Lin YC, Brayfield CA, Gerlach JC, Rubin JP, Marra KG, Acta Biomater., 5, 1416 (2009)
Soucy PA, Romer LH, Matrix Biol., 28, 273 (2009)
Zhang Y, He Y, Bharadwaj S, Hammam N, Carnagey K, Myers R, Atala A, Van Dyke M, Biomaterials, 30, 4021 (2009)
Narayanan K, Leck KJ, Gaoa S, Wan A, Biomaterials, 30, 4309 (2009)
Datta N, Pham QP, Sharma U, Sikavitsas VI, Jansen JA, Mikos AG, Proc. Natl. Acad. Sci., 103, 2488 (2006)
Cheng HW, Tusi YK, Cheung KM, Chan D, Chan BP, Tissue Eng. Part C: Methods, 15, 697 (2009)
Arinzeh TL, Tran T, Mcalary J, Daculsi G, Biomaterials, 26, 3631 (2005)
Philp D, Chen SS, Fitzerald W, Orenstein J, Margolis L, Kleinman HK, Stem Cells, 23, 288 (2005)

[Related Articles]

[1] Discher DE, Mooney DJ, Zandstra PW, Science, 324, 1673 (2009)

[2] Juliano RL, Haskill S, J. Cell Biol., 120, 577 (1993)

[3] Badylak SF, in Encyclopedia of Biomaterials and Biomedical Engineering, Wnek GE, Bowlin GL, Eds., Marcel Dekker Inc., New York, 2004, p 561.

[4] Ott HC, Matthiesen TS, Goh SK, Black LD, Kren SM, Netoff TI, Taylor DA, Nat. Med., 14, 213 (2008)

[5] Iop L, Renier V, Naso F, Piccoli M, Bonetti A, Gandaglia A, Pozzobon M, Ortolani F, Marchini M, Spina M, Coppip D, Sartore S, Gerosa G, Biomaterials, 30, 4104 (2009)

[6] Park SN, Kim JK, Suh H, Biomaterials, 25, 3689 (2004)

[7] Serban MA, Liu Y, Prestwich GD, Acta Biomater., 4, 67 (2008)

[8] Bhati RS, Mukherjee DP, McCarthy KJ, Rogers SH, Smith DF, Shalaby SW, J. Biomed. Mater. Res., 56, 74 (2001)

[9] Zhang H, Tasnim F, Ying YJ, Zink D, Biomaterials, 30, 2899 (2009)

[10] Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR, Science, 284, 143 (1999)

[11] Pelham RJ, Wang Y, Proc. Natl. Acad. Sci., 94, 13661 (1997)

[12] Lin YC, Brayfield CA, Gerlach JC, Rubin JP, Marra KG, Acta Biomater., 5, 1416 (2009)

[13] Soucy PA, Romer LH, Matrix Biol., 28, 273 (2009)

[14] Zhang Y, He Y, Bharadwaj S, Hammam N, Carnagey K, Myers R, Atala A, Van Dyke M, Biomaterials, 30, 4021 (2009)

[15] Narayanan K, Leck KJ, Gaoa S, Wan A, Biomaterials, 30, 4309 (2009)

[16] Datta N, Pham QP, Sharma U, Sikavitsas VI, Jansen JA, Mikos AG, Proc. Natl. Acad. Sci., 103, 2488 (2006)

[17] Cheng HW, Tusi YK, Cheung KM, Chan D, Chan BP, Tissue Eng. Part C: Methods, 15, 697 (2009)

[18] Arinzeh TL, Tran T, Mcalary J, Daculsi G, Biomaterials, 26, 3631 (2005)

[19] Philp D, Chen SS, Fitzerald W, Orenstein J, Margolis L, Kleinman HK, Stem Cells, 23, 288 (2005)