Biochemical and Biophysical Research Communications, Vol.407, No.3, 548-551, 2011
The sequence d(CGGCGGCCGC) self-assembles into a two dimensional rhombic DNA lattice
We report here the crystal structure of the partially self-complementary decameric sequence d(CGGCGGCCGC), which self assembles to form a four-way junction with sticky ends. Each junction binds to four others through Watson-Crick base pairing at the sticky ends to form a rhombic structure. The rhombuses bind to each other and form two dimensional tiles. The tiles stack to form the crystal. The crystal diffracted in the space group P1 to a resolution of 2.5 angstrom. The junction has the anti-parallel stacked-X conformation like other junction structures, though the formation of the rhombic net noticeably alters the details of the junction geometry. (C) 2011 Elsevier Inc. All rights reserved.