Development and Characterization of Nanofibrous Poly(lactic-co-glycolic acid)/Biphasic Calcium Phosphate Composite Scaffolds for Enhanced Osteogenic Differentiation

Ji-Hye Lee; Yu-Bin Lee; Nae-Gyune Rim; Sun-Young Jo; Youn-Mook Lim; Heungsoo Shin

Macromolecular Research, Vol.19, No.2, 172-179, February, 2011

DOI10.1007/s13233-011-0206-4 Export Citation

Development and Characterization of Nanofibrous Poly(lactic-co-glycolic acid)/Biphasic Calcium Phosphate Composite Scaffolds for Enhanced Osteogenic Differentiation

Ji-Hye Lee¹, Yu-Bin Lee¹, Nae-Gyune Rim¹, Sun-Young Jo², Youn-Mook Lim², and Heungsoo Shin^{1, 3, †}

¹Department of Bioengineering, Hanyang University, Seoul, 133-791, Korea
²Radiation Research Division for Industry & Environment, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeollabuk-do, 580-185, Korea
³Institute for Bioengineering and Biopharmaceutical Research, Seoul, 133-791, Korea

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Poly(lactic-co-glycolic acid)(PLGA)/biphasic calcium phosphate (BCP) composite nanofibers with different BCP to PLGA ratios were fabricated using the electrospinning technique. The scanning electron microscopy (SEM) images showed a similar morphology and fibers in all groups. The incorporated BCP was dispersed homogenously throughout the nanofibers, and the surface roughness was affected by the input amount of BCP. The increase in amount of BCP incorporated was confirmed by several methods. BCP incorporation into the PLGA nanofibers did not affect the initial adhesion of osteoblasts and their adherent morphology. However, the proliferation of the cells cultured on the composite nanofibers for 10 days with larger amounts of BCP was delayed, suggesting that incorporated BCP may facilitate the switch from proliferation to differentiation of the osteoblasts. The incorporation of BCP enhanced the expression of osteogenic genes, as well as induced calcium deposition by the osteoblasts in the extracellular matrix(ECM) after 21 days of culture on the PLGA/BCP composite nanofibers. Overall, these results can provide evidence of the potential of BCP incorporation into the biomaterials for effective bone regeneration.

Keywords:poly(lactic-co-glycolic acid);biphasic calcium phosphate;osteoblast;composite nanofibers.

[References]

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[2] El-Ghannam A, Exp. Rev. Med. Devic., 2, 87 (2005)

[3] Bohner M, Injury, 31 Supplement 4, D37 (2000).

[4] Jarcho M, Clin. Orthop. Rel. Res, 157, 259 (1981)

[5] Beck GR, Sullivan EC, Moran E, Zerler B, J. Cell. Biochem., 68, 269 (1998)

[6] Daculsi G, Legeros RZ, Nery E, Lynch K, Kerebel B, J. Biomed. Mater. Res., 23, 883 (1989)

[7] Daculsi G, LeGeros R, Heughebaert M, Barbieux I, Calcif. Tissue Int., 46, 20 (1990)

[8] Fujita R, Yokoyama A, Kawasaki T, Kohgo T, J. Oral Maxillofac. Surg., 61, 1045 (2003)

[9] Ryu HS, Youn HJ, Hong KS, Chang BS, Lee CK, Chung SS, Biomaterials, 23, 3 (2002)

[10] Daculsi G, Biomaterials, 19, 1473 (1998)

[11] Daculsi G, Laboux O, Malard O, Weiss P, J. Mater. Sci. -Mater. Med., 14, 195 (2003)

[12] Daculsi G, Uzel AP, Weiss P, Goyenvalle E, Aguado E, J. Mater. Sci. -Mater. Med., 21, 3 (2010)

[13] LeGeros RZ, Lin S, Rohanizadeh R, Mijares D, LeGeros JP, J. Mater. Sci. -Mater. Med., 14, 201 (2003)

[14] Saldana L, Sanchez-Salcedo S, Izquierdo-Barba I, Bensiamar F, Munuera L, Vallet-Regi M, Vilaboa N, Acta Biomater., 5, 1294 (2009)

[15] Zhang L, Hanagata N, Maeda M, Minowa T, Ikoma T, Fan H, Zhang X, Sci. Technol. Adv. Mater., 10, 2 (2009)

[16] Liu X, Ma PX, Ann. Biomed. Eng., 32, 477 (2004)

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[19] Song JH, Kim HE, Kim HW, J. Mater. Sci. -Mater. Med., 19, 8 (2008)

[20] Zhang Y, Venugopal JR, El-Turki A, Ramakrishna S, Su B, Lim CT, Biomaterials, 29, 32 (2008)

[21] Chen J, Chu B, Hsiao BS, J. Biomed. Mater. Res., 79, 307 (2006)

[22] McCullen SD, Zhu Y, Bernacki SH, Narayan RJ, Pourdeyhimi B, Gorga RE, Loboa EG, Bio-Med. Mater. Eng., 4, 3 (2009)

[23] Jeong SI, Lee AY, Lee YM, Shin H, J. Biomater. Sci.-Polym. Ed., 19, 339 (2008)

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[25] Alcaide M, Serrano MC, Pagani R, Sanchez-Salcedo S, Vallet-Regi M, Portoles MT, Biomaterials, 30, 45 (2009)

[26] Lian JB, Stein GS, Crit. Rev. Oral Biol. Med., 3, 269 (1992)

[27] Shin H, Zygourakis K, Farach-Carson MC, Yaszemski MJ, Mikos AG, J. Biomed. Mater. Res., 69, 3 (2004)

[28] Kulterer B, Friedl G, Jandrositz A, Sanchez-Cabo F, Prokesch A, Paar C, Scheideler M, Windhager R, Preisegger KH, Trajanoski Z, BMC Genomics, 8, 70 (2007)

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[30] Bernhardt A, Despang F, Lode A, Demmler A, Hanke T, Gelinsky M, J. Tissue Eng. Regen. Med., 3, 54 (2009)