Pulse Response Test in Medium Pressure Protein Chromatography

Kim IH; Kim JT

Korean Journal of Chemical Engineering, Vol.15, No.6, 575-579, November, 1998

An MPLC (Medium Pressure Liquid Chromatography) column was in-house packed with an anion exchange silica gel (PAE 1000, Amicon) in order to carry out pulse response experiments to investigate MPLC column efficiency. Bovine serum albumin was isocratically eluted from the column with varying ionic strength of NaCl, and the chromatograms obtained were analyzed by the moment method. Broadening of the elution band was caused mostly by a longitudinal dispersion in our system, and no significant numerical differences in HETP with the velocity change were observed at the high ionic strength.

Keywords:Moment Analysis;Medium Pressure;Protein Chromatography

[References]

Ahn SH, Chung IJ, Korean J. Chem. Eng., 2(1), 11 (1985)
Bungay HR, Belfort G, "Advanced Biochemical Engineering," John Wiley & Sons, New York (1987)
Ghim YS, "On the Performance of Liquid Chromatography for the Separation of Glucose and Fructose," MS Thesis, KAIST (1980)
Kim IH, Kim JT, Chung BH, Korean J. Appl. Microbioal. Biotechnol., 23(4), 485 (1995)
Kim IH, Yune SW, Lee SM, Song JY, Korean J. Biological Response Modifiers, 1(1), 105 (1991)
Ladish MR, Kohlmann KL, Biotechnol. Prog., 8, 469 (1992)
Liu HC, "Membrane-based Affinity Chromatography," Ph.D. Thesis, Univ. Cincinnati, p. 20 (1993)
Park IS, Suh GD, HWAHAK KONGHAK, 22(5), 279 (1984)
Sakamoto K, Bio-Industry, 4(9), 751 (1987)
Sisson WG, Begovich JM, Byers CH, Scott CD, Chem. Tech., Aug., 498 (1988)
Subramanian G, "Preparative and Process-Scale Liquid Chromatography," Ellis Horwood, New York (1991)
Wheelwright SM, "Protein Purification: Design and Scale-Up of Downstream Processing," Hanser, Munich (1991)
Yamamoto S, Nakanish K, Matsno R, "Ion-Exchange Chromatography of Proteins," Dekker, New York (1988)

[Referenced By]

[1] Ahn SH, Chung IJ, Korean J. Chem. Eng., 2(1), 11 (1985)

[2] Bungay HR, Belfort G, "Advanced Biochemical Engineering," John Wiley & Sons, New York (1987)

[3] Ghim YS, "On the Performance of Liquid Chromatography for the Separation of Glucose and Fructose," MS Thesis, KAIST (1980)

[4] Kim IH, Kim JT, Chung BH, Korean J. Appl. Microbioal. Biotechnol., 23(4), 485 (1995)

[5] Kim IH, Yune SW, Lee SM, Song JY, Korean J. Biological Response Modifiers, 1(1), 105 (1991)

[6] Ladish MR, Kohlmann KL, Biotechnol. Prog., 8, 469 (1992)

[7] Liu HC, "Membrane-based Affinity Chromatography," Ph.D. Thesis, Univ. Cincinnati, p. 20 (1993)

[8] Park IS, Suh GD, HWAHAK KONGHAK, 22(5), 279 (1984)

[9] Sakamoto K, Bio-Industry, 4(9), 751 (1987)

[10] Sisson WG, Begovich JM, Byers CH, Scott CD, Chem. Tech., Aug., 498 (1988)

[11] Subramanian G, "Preparative and Process-Scale Liquid Chromatography," Ellis Horwood, New York (1991)

[12] Wheelwright SM, "Protein Purification: Design and Scale-Up of Downstream Processing," Hanser, Munich (1991)

[13] Yamamoto S, Nakanish K, Matsno R, "Ion-Exchange Chromatography of Proteins," Dekker, New York (1988)