Biomimetic sequestration of carbon dioxide using an enzyme extracted from oyster shell

Dae-Hoon Kim; Mari Vinoba; Woo-Sup Shin; Kyong-Soo Lim; Soon-Kwan Jeong; Sung-Hyun Kim

Korean Journal of Chemical Engineering, Vol.28, No.10, 2081-2085, October, 2011

DOI10.1007/s11814-011-0071-z Export Citation

Biomimetic sequestration of carbon dioxide using an enzyme extracted from oyster shell

Dae-Hoon Kim^{1, 2}, Mari Vinoba², Woo-Sup Shin², Kyong-Soo Lim², Soon-Kwan Jeong^{2, †}, and Sung-Hyun Kim^{1, †}

¹Department of Chemical and Biological Engineering, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-701, Korea
²Korea Institute of Energy Research, 71-2, Jang-dong, Yuseong-gu, Daejeon 305-343, Korea

E-mail:,

Carbon dioxide sequestration activity was compared and evaluated using bovine carbonic anhydrase (BCA), and a water soluble protein extract derived from hemocytes from diseased shell (HDS). Para-nitrophenyl acetate (p-NPA) was used to measure the reaction rate. The kcat/Km values obtained from the Lineweaver-Burk and Michaelis-Menten equations were 230.7M.1s.1 for BCA and 194.1M.1s.1 for HDS. Without a biocatalyst, CaCO3 production took 15 seconds on average, while it took 5 seconds on average when BCA or HDS were present, indicating an approximately 3-fold enhancement of CaCO3 production rate by the biocatalysts. The biocatalytic hydration of CO2 and its precipitation as CaCO3 in the presence of biocatalysts were investigated.

Keywords:Carbon Dioxide;Sequestration;Bovine Carbonic Anhydrase;Hemocytes from Diseased Shell;Biomineralization

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Lee SW, Choi CS, Cryst. Growth Des., 7, 1463 (2007)
Vinoba M, Kim DH, Lim KS, Jeong SK, Lee SW, Alagar M, Energy Fuels., 25, 438 (2011)
Mirjafari P, Asghari K, Mahinpey N, Ind. Eng. Chem. Res., 46(3), 921 (2007)
Berman A, Addadi L, Weiner S, Nature., 331, 546 (1988)
Ray B, Cell. Mol. Life Sci., 33, 1439 (1977)

[Related Articles]

[1] NASA, Global Warming. Goddard Space Flight Center., 207711, 286 (1998)

[2] Metz B, Davidson O, de Coninck H, Loos M, Meyer L, IPCC Special Report on Carbon Capture and Storage (2005)

[3] Ramanathan V, Bulletin of the American Academy of Arts and Sciences., 36 (2006)

[4] OECD/IEA, Energy Technology Perspectives (2008)

[5] Saha AK, Biswas AK, Bandyopadhyay SS, Sep. Purif. Technol., 15(2), 101 (1999)

[6] Bishnoi S, Rochelle GT, Chem. Eng. Sci., 55(22), 5531 (2000)

[7] Ruihong Z, Fen G, Yongqi H, Huanqi Z, Micropor. Mesopor. Mater., 93, 212 (2006)

[8] Park YW, Baek IH, Park SD, Lee JW, Park SJ, Korean Chem. Eng. Res., 45(6), 573 (2007)

[9] Hwang YD, Shin HY, Kwak H, Bae SY, Korean Chem. Eng. Res., 44(6), 588 (2006)

[10] Bishnoi SG, Rochelle T, AIChE J., 48 (2002)

[11] Aineto M, Acosta A, Rincon JM, Romero M, Fuel., 85, 2352 (2006)

[12] Sullivan BP, Krist K, Guard HE, Electrical and electrocatalytic reactions of carbon dioxide, Elsevier, New York (1993)

[13] Ho C, Strurevant JM, J. Biol. Chem., 238, 1 (1963)

[14] Khalifah RC, J. Biol. Chem., 246, 2561 (1971)

[15] Mirjafari P, Asghari K, Mahinpey N, Ind. Eng. Chem. Res., 46(3), 921 (2007)

[16] Bond GM, Stringer J, Brandvold DK, Simsek FA, Medina MG, Egeland G, Energy Fuels, 15(2), 309 (2001)

[17] Lee SW, Choi CS, Cryst. Growth Des., 7, 1463 (2007)

[18] Vinoba M, Kim DH, Lim KS, Jeong SK, Lee SW, Alagar M, Energy Fuels., 25, 438 (2011)

[19] Mirjafari P, Asghari K, Mahinpey N, Ind. Eng. Chem. Res., 46(3), 921 (2007)

[20] Berman A, Addadi L, Weiner S, Nature., 331, 546 (1988)

[21] Ray B, Cell. Mol. Life Sci., 33, 1439 (1977)