REMOVAL OF HYDROGEN SULFIDE IN A THREE PHASE FLUIDIZED BED BIOREACTOR

Oh KJ; Seo MH; Son HJ; Kim D

Korean Journal of Chemical Engineering, Vol.15, No.2, 177-181, March, 1998

To remove hydrogen sulfide biologically, a three phase fluidized bed bioreactor was used in which Thiobacillus sp.IW was immobilized on biosands. The optimum operating condition of the bioreactor was found to be 30℃, pH 7, bed height of 0.85-1.0 m and aspect ratio of 1.0. At these conditions, the bioreactor removed more than 88 % of the hydrogen sulfide for an inlet concentration of 30-160 ppm and a gas flow rate of 2-5 l/m. The maximum removal rate obtained was about 1,000 mg H₂S/min. In continuous operation of the bioreactor, the removal efficiency remained 99 % for up to 16 hours and decreased to 91 % at 52 hours.

Keywords:Hydrogen Sulfide;Thiobacillus sp.IW;Immobilization;Three Phase Fluidized Bed Bioreactor;Removal Rate

[References]

Barth CL, Elliott FL, Melvin SW, Trans. ASCE, 27, 859 (1984)
Brock TD, Madigan MT, "Biology of Microorganisms," 6th Ed., Prentice-Hall, Englewood Cliffs, New Jersey (1991)
Cha JM, Park Y, Lee IW, Korean J. Biotechnol. Bioeng., 9, 287 (1994)
Chang CH, Shin NC, "The Engineering of Odor Control," Dong Wha, Seoul (1993)
Cho KS, Zang L, Hirai M, Shoda M, J. Ferment. Bioeng., 71, 44 (1991)
Chung YC, Huang CP, Tseng CP, Biotechnol. Prog., 12(6), 773 (1996)
Chung YC, Huang C, Tseng CP, Biotechnol. Tech., 10, 743 (1996)
Huang C, Chung YC, Hsu BM, Biotechnol. Tech., 10, 595 (1996)
Janssen AJ, Ma SC, Lens P, Lettinga G, Biotechnol. Bioeng., 53(1), 32 (1997)
Kim BW, Chang HN, Biotechnol. Prog., 7, 495 (1991)
Kim S, "A Study on Immobilization Characteristics of Thiobacillus Sp. IW on Activated Carbon," Masters Thesis, Pusan National University (1996)
Kim SM, Oh KH, Kim D, Korean J. Biotechnol. Bioeng., 11, 78 (1996)
Kusai K, Yamanaka T, Biochim. Biophys. Acta, 325, 304 (1973)
Ryu HW, Korean J. Biotechnol. Bioeng., 11, 238 (1996)
Son HJ, "A Study on Development of the Effective H2S Removing Equipment Using Thiobacillus Sp. IW," Masters Thesis, Pusan National University (1997)
Sublette KL, Sylvester ND, Biotechnol. Bioeng., 29, 753 (1987)
Tabita R, Lundgren DG, J. Bacteriol., 108, 328 (1971)
Tanji Y, Kanagawa T, Mikami E, J. Ferment. Bioeng., 67, 280 (1989)
Then J, Truper HG, Arch. Microbiol., 135, 254 (1983)
Yang SB, Lee SH, "The Composition Analysis of the Odor," Dong Wha, Seoul (1994)

[Referenced By]

[1] Barth CL, Elliott FL, Melvin SW, Trans. ASCE, 27, 859 (1984)

[2] Brock TD, Madigan MT, "Biology of Microorganisms," 6th Ed., Prentice-Hall, Englewood Cliffs, New Jersey (1991)

[3] Cha JM, Park Y, Lee IW, Korean J. Biotechnol. Bioeng., 9, 287 (1994)

[4] Chang CH, Shin NC, "The Engineering of Odor Control," Dong Wha, Seoul (1993)

[5] Cho KS, Zang L, Hirai M, Shoda M, J. Ferment. Bioeng., 71, 44 (1991)

[6] Chung YC, Huang CP, Tseng CP, Biotechnol. Prog., 12(6), 773 (1996)

[7] Chung YC, Huang C, Tseng CP, Biotechnol. Tech., 10, 743 (1996)

[8] Huang C, Chung YC, Hsu BM, Biotechnol. Tech., 10, 595 (1996)

[9] Janssen AJ, Ma SC, Lens P, Lettinga G, Biotechnol. Bioeng., 53(1), 32 (1997)

[10] Kim BW, Chang HN, Biotechnol. Prog., 7, 495 (1991)

[11] Kim S, "A Study on Immobilization Characteristics of Thiobacillus Sp. IW on Activated Carbon," Masters Thesis, Pusan National University (1996)

[12] Kim SM, Oh KH, Kim D, Korean J. Biotechnol. Bioeng., 11, 78 (1996)

[13] Kusai K, Yamanaka T, Biochim. Biophys. Acta, 325, 304 (1973)

[14] Ryu HW, Korean J. Biotechnol. Bioeng., 11, 238 (1996)

[15] Son HJ, "A Study on Development of the Effective H2S Removing Equipment Using Thiobacillus Sp. IW," Masters Thesis, Pusan National University (1997)

[16] Sublette KL, Sylvester ND, Biotechnol. Bioeng., 29, 753 (1987)

[17] Tabita R, Lundgren DG, J. Bacteriol., 108, 328 (1971)

[18] Tanji Y, Kanagawa T, Mikami E, J. Ferment. Bioeng., 67, 280 (1989)

[19] Then J, Truper HG, Arch. Microbiol., 135, 254 (1983)

[20] Yang SB, Lee SH, "The Composition Analysis of the Odor," Dong Wha, Seoul (1994)