Synthetic nanomagnetite has been suggested as a potential reactant for the in situ treatment of contaminated groundwater. Although the application of magnetite nanoparticles for environmental remediation is promising, a full understanding of particle reactivity has been deterred by the propensity of the nanoparticles to aggregate and become colloidally unstable, Attractive magnetic interactions between particles are partially responsible for their aggregation. In this study, we characterized the magnetic behavior of magnetite by determining the saturation magnetization, coercivity, remanent magnetization, susceptibility, and blocking temperature of synthetic magnetite using a superconducting quantum interference device (SQUID). We show how these properties vary in the presence of surface-associated solutes such as tetramethylammonium (TMA(1)) and ferrous (Fe-II) cations. More importantly, because magnetite readily reacts with O-2 to produce maghemite, we analyzed the effect of oxidation on the magnetic properties of the particles. Because maghemite has a reported magnetic saturation that is less than that of magnetite, we hypothesized that oxidation would decrease the magnitude of the magnetic attractive force between adjacent particles. The presence of TMA(4) and Fe-II caused si change in the magnetic properties of magnetite potentially because of alterations in its crystalline order. Magnetite oxidation caused a decrease M saturation magnetization, resulting in less significant magnetic interactions between particles. Oxidation, therefore, could lead to the decreased aggregation of magnetite nanoparticles and a potential enhancement of their colloidal stability.