In this letter, a pH-responsive reactivated biointerface is fabricated using an inclusion reaction between an azobenzene-containing self-assembled monolayer and pH-responsive poly(ethylene glycol)-block-poly(acrylic acid) grafted with cyclodextrins. The pH-responsive interface can be switched between an extended state and a relaxed state for the reversible resistance of cytochrome c adsorption completely in cooperation with protein-resistant poly(ethylene glycol).