Preparative Separation of Phospholipids from Soybean by NP-HPLC

Row KH; Lee JW

Korean Journal of Chemical Engineering, Vol.14, No.5, 412-415, September, 1997

Soybean phospholipids-phosphatidylethanolamine (PE), phosphatidylinositol (PI),, and phosphatidylcholine (PC)-were separated by normal-phase high performance liquid chromatography. The system was operated in a gradient mode. The experimental variables were gradient time and mobile phase composition. The experimental results showed that PE, PI and PC were resolved by three step-change gradient modes which employed ternary systems of hexane/1-propanol/water (58/40/2 and 56/40/4 by vol.%) and methanol/1-propanol/water (80/18/2, by vol.%) for the gradient times of 10, 30 and 76 min, respectively.

Keywords:Phospholipids;Soybean;Preparative Separation;HPLC

[References]

Amari JV, Brown PR, Grill CM, Turcotte JG, J. Chromatogr., 511, 219 (1990)
Balazs PE, Schmit PL, Szuhaj BF, J. Am. Oil Chem. Soc., 73, 193 (1996)
Blank ML, Snyder F, J. Chromatogr., 273, 415 (1983)
Gunstone FD, Harwood JL, Padley FB, "The Lipid Handbook," 2nd Ed., Chapman & Hall, London, 1 (1994)
Hanras C, Perrin JL, J. Am. Oil Chem. Soc., 68, 804 (1991)
Hurst WJ, Martin RA, Sheeley RM, J. Liq. Chrom., 9, 2969 (1986)
Meeren PV, J. Am. Oil Chem. Soc., 67, 815 (1990)
Meeren PV, Vanderdeelen J, Huys M, baert L, "Phospholipids," Plenum Press, New York, 273 (1990)
Meeren PV, Vanderdeelen J, Huyghebaert G, Baert L, Chromatographia, 34, 557 (1992)
Meulenaer BD, Meeren PV, Vanderdeelen J, Baert L, J. Am. Oil Chem. Soc., 72, 1073 (1995)
Meulenaer BD, Meeren PV, Vanderdeelen J, Baert L, Chromatographia, 41, 527 (1995)
Moreau RA, "Lipid Chromatographic Analysis," (Ed. Takayuki Shibamoto), Chromatographic Science Series, Marcel Dekker, Inc., New York, 65, 257 (1994)
Patton GM, Fasulo JM, Robins SJ, J. Lipid Res., 23, 190 (1982)

[Referenced By]

[1] Amari JV, Brown PR, Grill CM, Turcotte JG, J. Chromatogr., 511, 219 (1990)

[2] Balazs PE, Schmit PL, Szuhaj BF, J. Am. Oil Chem. Soc., 73, 193 (1996)

[3] Blank ML, Snyder F, J. Chromatogr., 273, 415 (1983)

[4] Gunstone FD, Harwood JL, Padley FB, "The Lipid Handbook," 2nd Ed., Chapman & Hall, London, 1 (1994)

[5] Hanras C, Perrin JL, J. Am. Oil Chem. Soc., 68, 804 (1991)

[6] Hurst WJ, Martin RA, Sheeley RM, J. Liq. Chrom., 9, 2969 (1986)

[7] Meeren PV, J. Am. Oil Chem. Soc., 67, 815 (1990)

[8] Meeren PV, Vanderdeelen J, Huys M, baert L, "Phospholipids," Plenum Press, New York, 273 (1990)

[9] Meeren PV, Vanderdeelen J, Huyghebaert G, Baert L, Chromatographia, 34, 557 (1992)

[10] Meulenaer BD, Meeren PV, Vanderdeelen J, Baert L, J. Am. Oil Chem. Soc., 72, 1073 (1995)

[11] Meulenaer BD, Meeren PV, Vanderdeelen J, Baert L, Chromatographia, 41, 527 (1995)

[12] Moreau RA, "Lipid Chromatographic Analysis," (Ed. Takayuki Shibamoto), Chromatographic Science Series, Marcel Dekker, Inc., New York, 65, 257 (1994)

[13] Patton GM, Fasulo JM, Robins SJ, J. Lipid Res., 23, 190 (1982)