Overcoming the inhibition effects of metal ions in the degradation of benzene and toluene by alcaligenes xylosoxidans Y234

Yeom SH; Yoo YJ

Korean Journal of Chemical Engineering, Vol.14, No.3, 204-208, May, 1997

Yeom SH, Yoo YJ

The effects of metal ions on the biodegradation of benzene and toluene were investigated. Among 12 tested metal ions, Cu²⁺, Ni²⁺, Co²⁺ and Ag⁺ inhibited the degradation of benzene and toluene severely by Alcaligenes xylosoxidans Y234. Cu²⁺ was found to inhibit catechol 1,2-dioxygenase in the degradation of benzene and toluene. Co²⁺ and Ni²⁺ were supposed to inhibit benzoate 1,2-dioxygenase, while Ag⁺ was supposed to inhibit benzaldehyde dehydrogenase in the degradation of toluene. The inhibition effect caused by these metal ions could be overcome both by microbial adaptation and by adding specific aromatic compounds to the broth.

Keywords:Metal ion;Benzene;Toluene;Biodegradation;Inhibition

[References]

Alexander M, "Biodegradation and Bioremediation," Academic Press, New York, 196 (1994)
Alvarez PJJ, Vogel TM, Appl. Environ. Microbiol., 57, 2981 (1991)
Arvin E, Jensen BK, Gundersen AT, Appl. Environ. Microbiol., 55, 3221 (1989)
Bradford MM, Anal. Biochem., 72, 248 (1976)
Chang MK, Thomas CV, Craig SC, Biotechnol. Bioeng., 41, 1057 (1993)
Chun WK, Genthner BRS, Appl. Environ. Microbiol., 62, 2317 (1996)
Duetz WA, deJong C, Williams PA, VanAndel JG, Appl. Environ. Microbiol., 60, 2858 (1994)
Gadd GM, Griffiths AJ, Microbiol. Ecol., 4, 303 (1978)
Gibson DT, Koch JR, Kallio RE, Biochemistry, 7, 2653 (1968)
Gottschalk G, "Bacterial Metabolism," 2nd ed., Springer-Verlag, New York, 1 (1985)
Hamzah RY, Al-Baharna BS, Appl. Microbiol. Biotechnol., 41, 250 (1994)
Hughes MN, Poole RK, "Metals and Micro-organisms," Chapman and Hall, London, 204 (1989)
Lee JY, Jung KH, Choi SH, Kim HS, Appl. Environ. Microbiol., 62, 2211 (1995)
Nakazawa T, Nakazawa A, Method Enzyme, 17A, 518 (1970)
Nazaki M, Method Enzyme, 17A, 522 (1970)
Oh YS, Shareefdeen Z, Baltzis BC, Bartha R, Biotechnol. Bioeng., 44(4), 533 (1994)
Satsangee R, Ghosh P, Appl. Microbiol. Biotechnol., 34, 127 (1990)
Waite JH, Tanzer ML, Anal. Biochem., 111, 131 (1981)
Yeh WK, Gibson DT, Liu TN, Biochem. Biophys. Res. Commun., 78, 401 (1977)
Yeom SH, Kim SH, Yoo YJ, Korean J. Chem. Eng., 14(1), 37 (1997)
Zylstra GJ, Gibson DT, "Aromatic Hydrocarbon Degradation: A Molecular Approach, Genetic Engineering," Plenum Press, New York, 183 (1991)

[Referenced By]

[1] Alexander M, "Biodegradation and Bioremediation," Academic Press, New York, 196 (1994)

[2] Alvarez PJJ, Vogel TM, Appl. Environ. Microbiol., 57, 2981 (1991)

[3] Arvin E, Jensen BK, Gundersen AT, Appl. Environ. Microbiol., 55, 3221 (1989)

[4] Bradford MM, Anal. Biochem., 72, 248 (1976)

[5] Chang MK, Thomas CV, Craig SC, Biotechnol. Bioeng., 41, 1057 (1993)

[6] Chun WK, Genthner BRS, Appl. Environ. Microbiol., 62, 2317 (1996)

[7] Duetz WA, deJong C, Williams PA, VanAndel JG, Appl. Environ. Microbiol., 60, 2858 (1994)

[8] Gadd GM, Griffiths AJ, Microbiol. Ecol., 4, 303 (1978)

[9] Gibson DT, Koch JR, Kallio RE, Biochemistry, 7, 2653 (1968)

[10] Gottschalk G, "Bacterial Metabolism," 2nd ed., Springer-Verlag, New York, 1 (1985)

[11] Hamzah RY, Al-Baharna BS, Appl. Microbiol. Biotechnol., 41, 250 (1994)

[12] Hughes MN, Poole RK, "Metals and Micro-organisms," Chapman and Hall, London, 204 (1989)

[13] Lee JY, Jung KH, Choi SH, Kim HS, Appl. Environ. Microbiol., 62, 2211 (1995)

[14] Nakazawa T, Nakazawa A, Method Enzyme, 17A, 518 (1970)

[15] Nazaki M, Method Enzyme, 17A, 522 (1970)

[16] Oh YS, Shareefdeen Z, Baltzis BC, Bartha R, Biotechnol. Bioeng., 44(4), 533 (1994)

[17] Satsangee R, Ghosh P, Appl. Microbiol. Biotechnol., 34, 127 (1990)

[18] Waite JH, Tanzer ML, Anal. Biochem., 111, 131 (1981)

[19] Yeh WK, Gibson DT, Liu TN, Biochem. Biophys. Res. Commun., 78, 401 (1977)

[20] Yeom SH, Kim SH, Yoo YJ, Korean J. Chem. Eng., 14(1), 37 (1997)

[21] Zylstra GJ, Gibson DT, "Aromatic Hydrocarbon Degradation: A Molecular Approach, Genetic Engineering," Plenum Press, New York, 183 (1991)