This study aimed to understand the relationship between gliding behavior of dislocations and reversed evolution of dislocation structures in pure body-centered cubic metals, using automotive-grade interstitial-free steels under strain ratio = 0 for case studies. Dislocation cells were converted into loop-patch structures, and the gilding behavior of dislocations would have changed from the original multiple-slips to single-slip when the maximum strain was reduced from 1.2 to 0.15 or 0.2%. The extent to which the reversed evolution of dislocation structures occurs is lower for maximum strain = 0.15% as compared to that of maximum strain = 0.2%. However, once the maximum strain is reduced to 0.1%, dislocation cells would persist, showing no signs of reversed evolution. A strain threshold for reversed evolution of dislocation structures was inferred to fall between maximum strain = 0.1% and maximum strain = 0.15%.